Protein carbonyls as a biomarker of foetal-neonatal hypoxic stress
| Autor: | Zuzana Chovanová, Martin Simko, Zdenka Durackova, Katarína Sumegová, Mikulas Chavko, Alexandra Maruniakova, Ingrid Zitnanova |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Clinical Biochemistry Trolox equivalent antioxidant capacity Oxidative phosphorylation medicine.disease_cause Antioxidants Protein Carbonylation chemistry.chemical_compound Internal medicine Mole medicine Humans Hypoxia Chromatography High Pressure Liquid Chemistry Infant Newborn Proteins Bilirubin General Medicine Hypoxia (medical) Fetal Blood Antioxidant capacity Endocrinology Biochemistry Trolox medicine.symptom Oxidation-Reduction Hypoxic stress Oxidative stress Biomarkers |
| Zdroj: | Clinical biochemistry. 40(8) |
| ISSN: | 0009-9120 |
| Popis: | Objectives: Investigation of the effect of hypoxic conditions during labour on the protein oxidative modifications and changes in plasma antioxidative capacity of newborns. Design and methods: Oxidative damage to proteins was determined by high-performance liquid chromatography. Antioxidative status was monitored by Trolox equivalent antioxidant capacity method. In our study, 11 hypoxic and 19 normoxic newborns were involved. Results: In hypoxic newborns, we have found a significant increase in protein carbonyl levels (3.55 ± 0.86 versus 3.24 ± 0.69 mol carbonyls/mol proteins, p = 0.045) and plasma antioxidant capacity (1.76 ± 0.056 versus 1.68 ± 0.097 mmol Trolox/L, p = 0.004) when compared to normoxic children. Bilirubin levels were unchanged ( p = 0.87). Conclusion: Our results show elevated levels of carbonyls in hypoxic neonates compared to normoxic children. The oxidative damage to proteins is not sufficiently prevented by increased antioxidant capacity detected in plasma of hypoxic newborns. |
| Databáze: | OpenAIRE |
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