Development of a Specific Anti-capsid Antibody- and Magnetic Bead-Based Immunoassay to Detect Human Norovirus Particles in Stool Samples and Spiked Mussels via Flow Cytometry
| Autor: | Louisa F. Ludwig-Begall, Benjamin G Dewals, Etienne Thiry, Mamadou Amadou Diallo, Ravo M. Razafimahefa, Alain Vanderplasschen, Caroline Deketelaere, Olivier Nivelles, Axel Mauroy |
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| Rok vydání: | 2021 |
| Předmět: |
Epidemiology
viruses Health Toxicology and Mutagenesis ved/biology.organism_classification_rank.species medicine.disease_cause Virus Flow cytometry Mice fluids and secretions Virology medicine Animals Humans Detection limit Immunoassay Chromatography biology medicine.diagnostic_test Chemistry ved/biology Magnetic Phenomena Norovirus virus diseases Flow Cytometry Bivalvia Capsid biology.protein Antibody Food Science Murine norovirus |
| Zdroj: | Food and environmental virology. 13(4) |
| ISSN: | 1867-0342 |
| Popis: | Human noroviruses impose a considerable health burden globally. Here, a flow cytometry approach designed for their detection in biological waste and food samples was developed using antibody-coated magnetic beads. Antipeptide antibodies against murine norovirus and various human norovirus genotypes were generated for capture and coated onto magnetic beads. A flow cytometry assay was then implemented to detect bead-bound human norovirus GI.3 in patient stool samples and in norovirus-spiked mussel digestive tissues. The detection limit for stool samples was 105 gc/mL, thus bettering detection limits of commercially available norovirus diagnosis quick kits of 100-fold; the detection limit in spiked mussels however was ten-fold higher than in stool samples. Further assays showed a decrease in fluorescence intensity for heat- or UV-inactivated virus particles. Overall, we demonstrate the application of a flow cytometry approach for direct detection of small non-enveloped virus particles such as noroviruses. An adaptation of the technology to routine diagnostics has the potential to contribute a rapid and sensitive tool to norovirus outbreak investigations. Further improvements to the method, notably decreasing the detection limit of the approach, may allow the analysis of naturally contaminated food and environmental samples. |
| Databáze: | OpenAIRE |
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