Moyamoya disease patient mutations in the RING domain of RNF213 reduce its ubiquitin ligase activity and enhance NFκB activation and apoptosis in an AAA+ domain-dependent manner
| Autor: | Shohab Youssefian, Akio Koizumi, Tsunehiro Mizushima, Yuki Oichi, Tohru Tezuka, Shigeru Taketani, Kouji H. Harada, Jungmi Choi, Midori Takeda, Minsoo Kim, Hatasu Kobayashi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
AAA Domain Ubiquitin-Protein Ligases Biophysics Apoptosis Ubiquitin-conjugating enzyme Biochemistry Moyamoya disease 03 medical and health sciences 0302 clinical medicine Ubiquitin medicine Humans Ring domain Amino Acid Sequence Polyubiquitin Molecular Biology Adenosine Triphosphatases biology RNF213 Chemistry Lysine HEK 293 cells NF-kappa B Ubiquitination Cell Biology medicine.disease Ubiquitin ligase Cell biology 030104 developmental biology HEK293 Cells 030220 oncology & carcinogenesis Domain (ring theory) Mutation Ubiquitin-Conjugating Enzymes biology.protein Mutant Proteins RING Finger Domains Transcription Factors NFκB |
| Zdroj: | Biochemical and Biophysical Research Communications. 525(3):668-674 |
| ISSN: | 0006-291X |
| Popis: | Moyamoya disease (MMD) is a cerebrovascular disease characterized by progressive occlusion of the internal carotid arteries. Genetic studies originally identified RNF213 as an MMD susceptibility gene that encodes a large 591 kDa protein with a functional RING domain and dual AAA+ ATPase domains. As the functions of RNF213 and its relationship to MMD onset are unknown, we set out to characterize the ubiquitin ligase activity of RNF213, and the effects of MMD patient mutations on these activities and on other cellular processes. In vitro ubiquitination assays, using the RNF213 RING domain, identified Ubc13/Uev1A as a key ubiquitin conjugating enzyme that together generate K63-linked polyubiquitin chains. However, nearly all MMD patient mutations in the RING domain greatly reduced this activity. When full-length proteins were overexpressed in HEK293T cells, patient mutations that abolished the ubiquitin ligase activities conversely enhanced nuclear factor κB (NFκB) activation and induced apoptosis accompanied with Caspase-3 activation. These induced activities were dependent on the RNF213 AAA+ domain. Our results suggest that the NFκB- and apoptosis-inducing functions of RNF213 may be negatively regulated by its ubiquitin ligase activity and that disruption of this regulation could contribute towards MMD onset. |
| Databáze: | OpenAIRE |
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