Immunohistochemical analysis of the expression of cellular transcription NFκB (p65), AP-1 (c-Fos and c-Jun), and JAK/STAT in leprosy
| Autor: | Tinara Leila de Souza Aarão, Kelly Emi Hirai, Jorge Rodrigues de Sousa, Luciana Mota Silva, Juarez de Souza, Francisca Regina Oliveira Carneiro, Hellen Thais Fuzii, Leônidas Braga Dias, Juarez Antônio Simões Quaresma |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_treatment
Lymphocyte Activation Pathology and Forensic Medicine Leprosy medicine Humans STAT1 STAT3 Mycobacterium leprae STAT4 Transcription factor Antigens Bacterial Immunity Cellular biology c-jun NF-kappa B JAK-STAT signaling pathway Janus Kinase 2 STAT4 Transcription Factor biology.organism_classification Molecular biology Transcription Factor AP-1 STAT1 Transcription Factor Cytokine Immunology biology.protein |
| Zdroj: | Human Pathology. 46:746-752 |
| ISSN: | 0046-8177 |
| Popis: | Leprosy is a disease whose clinical spectrum depends on the cytokine patterns produced during the early stages of the immune response. The main objective of this study was to describe the activation pattern of cellular transcription factors and to correlate these factors with the clinical forms of leprosy. Skin samples were obtained from 16 patients with the tuberculoid (TT) form and 14 with the lepromatous (LL) form. The histologic sections were immunostained with anti-c-Fos and anti-c-Jun monoclonal antibodies for investigation of AP-1, anti-NFκB p65 for the study of NFκB, and anti-JAK2, STAT1, STAT3, and STAT4 for investigation of the JAK/STAT pathway. Cells expressing STAT1 were more frequent in the TT form than in LL lesions (P = .0096), in agreement with the protective immunity provided by IFN-γ. STAT4 was also more highly expressed in the TT form than in the LL form (P = .0098). This transcription factor is essential for the development of a Th1 response because it is associated with interleukin-12. NFκB (p65) and STAT4 expression in the TT form showed a strong and significant correlation (r = 0.7556 and P = .0007). A moderate and significant correlation was observed between JAK2 and STAT4 in the TT form (r = 0.6637 and P = .0051), with these factors responding to interleukin-12 in Th1 profiles. The results suggest that STAT1, JAK2, and NFκB, together with STAT4, contribute to the development of cell-mediated immunity, which is able to contain the proliferation of Mycobacterium leprae. |
| Databáze: | OpenAIRE |
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