The effectiveness of Hedyotis diffusa Willd extract in a mouse model of experimental autoimmune prostatitis
| Autor: | Rahat Ullah, Nasrin Aktar, Muhammad Waqar Khan, Xingxing Cui, Xiaohui Zhou, Amir Hossain, Junaid Wazir, Puregmaa Khongorzul |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Urology medicine.medical_treatment media_common.quotation_subject 030232 urology & nephrology Prostatitis Traditional Chinese medicine Pelvic Pain Gastroenterology Urination Hedyotis diffusa Mice 03 medical and health sciences 0302 clinical medicine Endocrinology Prostate Internal medicine Threshold of pain Hedyotis medicine Animals Humans Saline media_common 030219 obstetrics & reproductive medicine biology business.industry General Medicine medicine.disease biology.organism_classification Staining Mice Inbred C57BL medicine.anatomical_structure Quality of Life business |
| Zdroj: | Andrologia. 53 |
| ISSN: | 1439-0272 0303-4569 |
| DOI: | 10.1111/and.13913 |
| Popis: | Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a frustrating and often debilitating disease. Current studies have shown that Traditional Chinese Medicine (TCM) can improve patients' quality of life and alleviate CP/CPPS symptoms. In this study, the efficacy of Hedyotis diffusa Willd aqueous extraction in experimental autoimmune prostatitis (EAP) mice models was revealed. The C57BL/6 mice were randomly assigned to three groups. Except for the control group, all other groups were subcutaneously injected with 0.2 ml emulsion of T2 peptide, on day 0 and day 14, for inducing EAP models. After the EAP modelling, oral saline was given to the model group, while the H. diffusa group was treated with aqueous extract of H. diffusa Willd. Micturition habits and withdrawal response frequencies were measured. Haematoxylin and eosin staining and immunohistochemistry were used to investigate inflammatory cell infiltration and TNF-α in the prostate tissue respectively. TNF-α levels in the serum were evaluated by ELISA. The H. diffusa Willd aqueous extraction considerably reduced the urine spots number and increased the pain threshold in H. diffusa group. H. diffusa group showed significantly reduced inflammatory lesion and inflammatory cell infiltration than the model group. The levels of TNF-α in H. diffusa group were considerably reduced. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|