Trophectoderm biopsy protocols may impact the rate of mosaic blastocysts in cycles with pre-implantation genetic testing for aneuploidy

Autor: Jiang Wang, Dongyun Liu, Jingyu Li, Guoning Huang, Shun Xiong, Yang Gao, Lihong Wu, Wei Han, Xiangwei Hao, Junxia Liu, Weiwei Liu, Jiahong Zhu
Rok vydání: 2021
Předmět:
0301 basic medicine
Pregnancy Rate
Biopsy
Aneuploidy
Biology
Andrology
03 medical and health sciences
0302 clinical medicine
Pregnancy
Ectoderm
Genetics
medicine
Blastocyst biopsy protocol
Humans
Embryo Implantation
Genetic Testing
Blastocyst
Assisted Reproduction Technologies
Zona pellucida
Preimplantation Diagnosis
reproductive and urinary physiology
Genetics (clinical)
Genetic testing
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
Mosaicism
Incidence (epidemiology)
Obstetrics and Gynecology
Retrospective cohort study
Embryo
General Medicine
Embryo Transfer
medicine.disease
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
Pre-implantation genetic testing for aneuploidies (PGT-A)
embryonic structures
Next-generation sequencing
Female
Developmental Biology
Zdroj: Journal of Assisted Reproduction and Genetics
ISSN: 1573-7330
1058-0468
DOI: 10.1007/s10815-021-02137-w
Popis: Purpose This study aimed to analyze the impact of different biopsy protocols on the rate of mosaic blastocysts. Methods This is a retrospective cohort study which included 115 cycles with pre-implantation genetic testing for aneuploidy (PGT-A). Two groups were allocated based on the biopsy protocols: method 1 group, the zona pellucida (ZP) was drilled on day 3 embryos followed by trophectoderm (TE) biopsy; and method 2 group, the ZP was opened on day 5 or 6 blastocysts followed by TE biopsy. All biopsy samples were assessed using next-generation sequencing (NGS) at a single reference laboratory. The euploid, aneuploid, and mosaic blastocyst rates and clinical outcomes were compared. Results The mosaicism rate in the method 1 group was 19.58%, significantly higher than the method 2 group (8.12%; P < 0.05). No statistically significant difference was observed in euploid, aneuploid blastocyst rates, and clinical pregnancy rates between the two groups. Logistic regression analysis indicated that the biopsy protocols were independently associated with the mosaicism rates among all the variables. Conclusions The present study showed that different biopsy protocols may have an impact on the mosaic blastocyst rate. ZP opening on day 3 combined with TE biopsy might increase the incidence of mosaic blastocysts.
Databáze: OpenAIRE
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