Satellite DNAs Unveil Clues about the Ancestry and Composition of B Chromosomes in Three Grasshopper Species
| Autor: | Ana B. S. M. Ferretti, Diogo Milani, Adriana de Souza Melo, Diogo Cavalcanti Cabral-de-Mello, Vanessa Bellini Bardella, Rita de Cássia de Moura, Octavio M. Palacios-Gimenez, Hojun Song, Vilma Loreto |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Uppsala University, UPE—Universidade de Pernambuco, Universidade Federal de Pernambuco (UFPE), 2475 TAMU |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine medicine.medical_specialty lcsh:QH426-470 Satellite DNA Orthoptera supernumerary chromosome satellite DNA Biology 010603 evolutionary biology 01 natural sciences Genome Intraspecific competition Article Fluorescent in situ hybridization Evolutionsbiologi 03 medical and health sciences Supernumerary chromosome Genetics medicine RepeatExplorer Grasshopper Repeatexplorer Genetics (clinical) B chromosome Evolutionary Biology Autosome Cytogenetics biology.organism_classification lcsh:Genetics 030104 developmental biology Evolutionary biology fluorescent in situ hybridization |
| Zdroj: | Genes, Vol 9, Iss 11, p 523 (2018) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Genes Volume 9 Issue 11 |
| ISSN: | 2073-4425 |
| Popis: | Made available in DSpace on 2019-10-06T15:24:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-11-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Supernumerary (B) chromosomes are dispensable genomic elements occurring frequently among grasshoppers. Most B chromosomes are enriched with repetitive DNAs, including satellite DNAs (satDNAs) that could be implicated in their evolution. Although studied in some species, the specific ancestry of B chromosomes is difficult to ascertain and it was determined in only a few examples. Here we used bioinformatics and cytogenetics to characterize the composition and putative ancestry of B chromosomes in three grasshopper species, Rhammatocerus brasiliensis, Schistocerca rubiginosa, and Xyleus discoideus angulatus. Using the RepeatExplorer pipeline we searched for the most abundant satDNAs in Illumina sequenced reads, and then we generated probes used in fluorescent in situ hybridization (FISH) to determine chromosomal position. We used this information to infer ancestry and the events that likely occurred at the origin of B chromosomes. We found twelve, nine, and eighteen satDNA families in the genomes of R. brasiliensis, S. rubiginosa, and X. d. angulatus, respectively. Some satDNAs revealed clustered organization on A and B chromosomes varying in number of sites and position along chromosomes. We did not find specific satDNA occurring in the B chromosome. The satDNAs shared among A and B chromosomes support the idea of putative intraspecific ancestry from small autosomes in the three species, i.e., pair S11 in R. brasiliensis, pair S9 in S. rubiginosa, and pair S10 in X. d. angulatus. The possibility of involvement of other chromosomal pairs in B chromosome origin is also hypothesized. Finally, we discussed particular aspects in composition, origin, and evolution of the B chromosome for each species. Instituto de Biociências/IB Departamento de Biologia UNESP—Universidade Estadual Paulista Department of Evolutionary Biology Evolutionary Biology Center Uppsala University Instituto de Ciências Biológicas Laboratório de Biodiversidade e Genética de Insetos UPE—Universidade de Pernambuco Centro de Biociências/CB Departamento de Genética UFPE—Universidade Federal de Pernambuco Department of Entomology Texas A&M University 2475 TAMU Instituto de Biociências/IB Departamento de Biologia UNESP—Universidade Estadual Paulista FAPESP: 2015/16661-1 CNPq: 305300/2017-2 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|