Properties and analysis of a stable derivative of pyrroline-5-car☐ylic acid for use in metabolic studies
| Autor: | Vasek A. Mezl, W. Eugene Knox |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
chemistry.chemical_classification
Chromatography Paper Carboxylic acid Carboxylic Acids Hydrazones Biophysics Periodate Cell Biology Glutamic acid Pyrroline Metabolic intermediate Borohydride Hydroxylysine Biochemistry Amino acid Kinetics chemistry.chemical_compound Glutamates chemistry Spectrophotometry Methods Organic chemistry Pyrroles Molecular Biology Derivative (chemistry) |
| Zdroj: | Analytical Biochemistry. 74:430-440 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(76)90223-2 |
| Popis: | The unstable metabolic intermediate 1Δ-pyrroline-5-car☐ylic acid (P5C or glutamic semialdehyde) cannot be isolated as a chemically pure solid because of its irreversible and concentration-dependent reaction with itself. This has been avoided by preparing it in dilute solution by periodate oxidation of dl-hydroxylysine and isolating it as a pure 2,4-dinitrophenylhydrazone from which P5C can be quantitatively regenerated. The product has been characterized by various chemical and enzymatic tests and by comparison with two other types of preparation of P5C. One of the reactions used to standardize it, reduction to proline by borohydride, also produced hydroxynorvaline in amounts indicating that an equilibrium exists between the ring and open forms of P5C and glutamic semialdehyde. |
| Databáze: | OpenAIRE |
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