Spatial frequency metrics for analysis of microscopic images of musculoskeletal tissues
| Autor: | Jarrod A. Call, Luke J. Mortensen, W. Michael Southern, Emily G. Pendleton, Kayvan Forouhesh Tehrani |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Genetically modified mouse
0206 medical engineering 02 engineering and technology Biology Biochemistry Article Mice 03 medical and health sciences Rheumatology Microscopy medicine Animals Orthopedics and Sports Medicine Muscle Skeletal Molecular Biology 030304 developmental biology 0303 health sciences Myogenesis Regeneration (biology) Shg microscopy Skeletal muscle Cell Biology Flat bone 020601 biomedical engineering Extracellular Matrix Benchmarking medicine.anatomical_structure Collagen Spatial frequency Biomedical engineering |
| Zdroj: | Connect Tissue Res |
| ISSN: | 1607-8438 0300-8207 |
| DOI: | 10.1080/03008207.2020.1828381 |
| Popis: | PURPOSE : Imaging-based metrics for analysis of biological tissues are powerful tools that can extract information such as shape, size, periodicity, and many other features to assess the requested qualities of a tissue. Muscular and osseous tissues consist of periodic structures that are directly related to their function, and so analysis of these patterns likely reflects tissue health and regeneration. METHODS : A method for assessment of periodic structures is by analyzing them in the spatial frequency domain using the Fourier transform. In this paper, we present two filters which we developed in the spatial frequency domain for the purpose of analyzing musculoskeletal structures. These filters provide information about 1) the angular orientation of the tissues and 2) their periodicity. We explore periodic structural patterns in the mitochondrial network of skeletal muscles that are reflective of muscle metabolism and myogenesis; and patterns of collagen fibers in the bone that are reflective of the organization and health of bone extracellular matrix. RESULTS: We present an analysis of mouse skeletal muscle in healthy and injured muscles. We used a transgenic mouse that ubiquitously expresses fluorescent protein in their mitochondria and performed 2-photon microscopy to image the structures. To acquire the collagen structure of the bone we used non-linear SHG microscopy of mouse flat bone. We analyze and compare juvenile versus adult mice, which have different structural patterns. CONCLUSIONS: Our results indicate that these metrics can quantify musculoskeletal tissues during development and regeneration. |
| Databáze: | OpenAIRE |
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