Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice
| Autor: | Myrielle Dupont-Rouzeyrol, Veasna Duong, Sébastien Boyer, Borin Peng, Philippe Dussart, Rithy Choeung, Tey Putita Ou, Heidi Auerswald, Senglong Pang, Saraden In |
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| Přispěvatelé: | Unité de Virologie / Virology Unit [Phnom Penh], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Medical and Veterinary Entomology - Entomologie médciale et vétérinaire [Phnom Penh, Cambodia], Dengue et Arbovirose (URE-DA), Institut Pasteur de Nouvelle-Calédonie, This work was supported by Institut Pasteur in Paris in the frame of the Actions Concertées Inter Pasteuriennes (ZikAe project, ACIP A-15-2014) and by the Institut Pasteur du Cambodge. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) African lineage Lineage (genetic) QH301-705.5 030106 microbiology Population Aedes aegypti Asian lineage Microbiology Article Zika virus 03 medical and health sciences Virology Biology (General) education neonatal mouse infection education.field_of_study vector competence biology Transmission (medicine) fungi Blood meal biology.organism_classification 3. Good health 030104 developmental biology Cell culture Vector (epidemiology) [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| Zdroj: | Microorganisms Microorganisms, MDPI, 2021, 9 (6), pp.1250. ⟨10.3390/microorganisms9061250⟩ Volume 9 Issue 6 Microorganisms, Vol 9, Iss 1250, p 1250 (2021) |
| ISSN: | 2076-2607 |
| Popis: | Since the epidemic in 2007, studies on vector competence for Zika virus (ZIKV) have intensified, showing that the transmission efficiency varies depending on the vector population, ZIKV strain, and dose of the infectious blood meal. In this study, we aimed to investigate the replication of African and Asian ZIKV strains in vitro and in vivo in order to reveal their phenotypic differences. In addition, we investigated the vector competence of Cambodian Aedes aegypti (Ae. aegypti) mosquitoes (urban and rural) for these ZIKV strains. We observed a significantly higher pathogenicity of the African ZIKV strain in vitro (in mosquito and mammalian cells), and in vivo in both Ae. aegypti and mice. Both mosquito populations were competent to transmit ZIKV as early as 7 days p.i., depending on the population and the ZIKV strain. Ae. aegypti from rural habitats showed significant higher transmission and survival rates than those from urban. We observed the highest transmission efficiency for the African ZIKV isolate (93.3% 14 days p.i.) and for the Cambodian ZIKV isolate (80% 14 days p.i.). Overall, our results highlight the phenotypic differences of the ZIKV lineages and the potential risk of ZIKV transmission by Ae. aegypti mosquitoes. Further investigations of Cambodian mosquito species and ZIKV specific surveillance in humans is necessary in order to improve the local risk assessment. |
| Databáze: | OpenAIRE |
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