Effects of glycation on human γd-crystallin proteins by different glycation-inducing agents
| Autor: | Chun-Hsien Lo, Steven S.-S. Wang, Chih-Kai Chang, Su-Chun How, Chien-Ting Li, Mei-Er Chen, Wei-An Chen, Josephine W. Wu, Min-Chih Chun |
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| Rok vydání: | 2018 |
| Předmět: |
Glycation End Products
Advanced 0301 basic medicine Protein Denaturation Glycosylation Ribose Lysine Protein aggregation Protein Aggregation Pathological Biochemistry Cataract Diabetes Complications 03 medical and health sciences chemistry.chemical_compound Cataracts Structural Biology Crystallin Glycation Lens Crystalline medicine Humans gamma-Crystallins Molecular Biology 030102 biochemistry & molecular biology Methylglyoxal Galactose General Medicine Pyruvaldehyde medicine.disease eye diseases 030104 developmental biology Fructosamine chemistry sense organs |
| Zdroj: | International Journal of Biological Macromolecules. 118:442-451 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.06.108 |
| Popis: | Human γ d -crystallin (Hγ d -crystallin), a major protein component of the human eye lens, is associated with the development of juvenile- and mature-onset cataracts. Evidence suggests that nonenzymatic protein glycation plays an important role in the aetiology of cataract and diabetic sequelae. This research compared the effects of various glycation modifiers on Hγ d -crystallin aggregation, by treating samples of Hγ d -crystallin with ribose, galactose, or methylglyoxal using several biophysical techniques. To measure advanced glycation end products, an Ne-(carboxyethyl)lysine enzyme-linked immunosorbent assay was performed on the glycating agent-treated Hγ d -crystallin samples. Fructosamine production detection was performed for both ribose-treated and galactose-treated samples. Methylglyoxal-treated samples had the highest level of aggregation and the greatest extent of unfolding, and upon incubation for a minimum of 12 days, exhibited a marked enhancement in the amount of Ne-(carboxyethyl)lysine. The molecular profiles and morphological features of the glycated samples were highly correlated to the type of glycation agent used. These findings highlight a close connection between the type of glycation modifier and the various aggregation species that form. Thus, these results may facilitate deciphering of the molecular mechanism of diabetic cataractogenesis. |
| Databáze: | OpenAIRE |
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