Bioelectrochemistry of microperoxidases
| Autor: | V.J. Razumas, T. Ruzgas, J. Kazlauskaite, Juozas J. Kulys |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Hemeprotein Chemistry Stereochemistry General Chemical Engineering Biophysics Peptide Electrochemistry Combinatorial chemistry Analytical Chemistry symbols.namesake chemistry.chemical_compound Adsorption Bioelectrochemistry medicine symbols Physical and Theoretical Chemistry Raman spectroscopy Heme Histidine |
| Zdroj: | Journal of Electroanalytical Chemistry. 343:159-176 |
| ISSN: | 1572-6657 |
| DOI: | 10.1016/0022-0728(92)85084-g |
| Popis: | This paper reports on an experimental study of the structure and functioning of heme-containing polypeptides (microperoxidases-8, -9 and -11) at the solid-electrolyte interface. The electrochemical and electrocatalytical properties of microperoxidases were investigated by electrochemical methods applying different electrode materials, whereas the adsorption and structure of these polypeptides were studied ellipsometrically and using surface-enhanced Raman spectroscopy. From the results obtained it was found that microperoxidases are useful models for investigating the interfacial behaviour of heme-containing proteins. The low molecular weight of the peptide chain and high exposure of the heme groups are the principal determinants for the observed direct electrochemical reactions. The influence of l -histidine coordination, the amino acid sequence of the polypeptide chain and surface hydrophobic-hydrophilic properties on the electrochemical and interfacial behaviour of microperoxidases were investigated. |
| Databáze: | OpenAIRE |
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