Evaluation of percutaneous absorption of esculetin: effect of chemical enhancers
| Autor: | Encarna Castillo García, Virginia Merino, Andrés Femenía Font, César Eulogio Serna Jiménez, María Aracely Calatayud Pascual, Sergio del Rio Sancho, Cristina Balaguer Fernández, Alicia C. López Castellano |
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| Rok vydání: | 2012 |
| Předmět: |
Time Factors
Swine Skin Absorption Pharmaceutical Science Absorption (skin) Pharmacology Sensitivity and Specificity Antioxidants Permeability Analytical Chemistry chemistry.chemical_compound Drug Delivery Systems Drug Stability Drug Discovery Cyclohexenes Animals Umbelliferones Ear External Enhancer Chromatography High Pressure Liquid Transdermal Skin Ethanol integumentary system Terpenes Laurocapram Organic Chemistry Reproducibility of Results Azepines Oleic acid Complementary and alternative medicine chemistry Percutaneous absorption Calibration Molecular Medicine Decenoic Acid Decanoic Acids Limonene Oleic Acid |
| Zdroj: | Planta medica. 79(2) |
| ISSN: | 1439-0221 |
| Popis: | Percutaneous transdermal absorption of esculetin (6,7-dihydroxycoumarin), an oxidative damage inhibitor, was evaluated by means of in vitro permeation studies in which vertical Franz-type diffusion cells and pig ear skin were employed. To determine the absorption of esculetin, we validated a simple, accurate, precise, and rapid HPLC-UV method. Additionally, the effects of several percutaneous enhancers were studied. Pretreatment of porcine skin was performed with ethanol (control vehicle), decenoic acid, oleic acid, R-(+)-limonene, and laurocapram (Azone®) (5 % in ethanol, w/w, respectively). Pretreatment of skin with oleic acid or laurocapram led to statistically significant differences in the transdermal flux of esculetin with respect to controls. Of the two enhancers, laurocapram showed the greatest capacity to enhance the flux of esculetin across pig skin. |
| Databáze: | OpenAIRE |
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