Genes involved in pre-mRNA 3′-end formation and transcription termination revealed by a lin-15 operon Muv suppressor screen
| Autor: | Alison Larsen, Mingxue Cui, Thomas Blumenthal, Margaret MacMorris, Min Han, Mary A. Allen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cleavage factor
Transcription Genetic Termination factor RNA polymerase II Cleavage and polyadenylation specificity factor Transcription (biology) Operon RNA Precursors Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Transcription factor Terminator Regions Genetic Genetics Cleavage stimulation factor Multidisciplinary biology Cleavage And Polyadenylation Specificity Factor RNA-Binding Proteins Biological Sciences Terminator (genetics) Cleavage Stimulation Factor biology.protein RNA Polymerase II RNA 3' End Processing Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 105:16665-16670 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0807104105 |
| Popis: | RNA polymerase II (Pol II) transcription termination involves two linked processes: mRNA 3′-end formation and release of Pol II from DNA. Signals for 3′ processing are recognized by a protein complex that includes cleavage polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF). Here we identify suppressors encoding proteins that play roles in processes at the 3′ ends of genes by exploiting a mutation in which the 3′ end of another gene is transposed into the first gene of the Caenorhabditis elegans lin-15 operon. As expected, genes encoding CPSF and CstF were identified in the screen. We also report three suppressors encoding proteins containing a domain that interacts with the C-terminal domain of Pol II (CID). We show that two of the CID proteins are needed for efficient 3′ cleavage and thus may connect transcription termination with RNA cleavage. Furthermore, our results implicate a serine/arginine-rich (SR) protein, SRp20, in events following 3′-end cleavage, leading to termination of transcription. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|