Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysis
| Autor: | Gittith Sánchez, Werner Apt, S Solari, Ximena Coronado, Marta Gajardo, Jose G. Venegas, Inés Zulantay |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Chagas disease
Adult Male flow cytometry anti-live trypomastigote antibodies (FC-ALTA) Itraconazole Veterinary (miscellaneous) Allopurinol Trypanosoma cruzi Antiprotozoal Agents Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Sensitivity and Specificity Serology law.invention Flow cytometry lcsh:Infectious and parasitic diseases law medicine Animals Humans Chagas Disease lcsh:RC109-216 Chile Fluorescent Antibody Technique Indirect Polymerase chain reaction biology medicine.diagnostic_test business.industry Reproducibility of Results medicine.disease biology.organism_classification Flow Cytometry Infectious Diseases Treatment Outcome Insect Science Immunology polymerase chain reaction (PCR) Animal Science and Zoology Parasitology Female chronic Chagas disease business Trypanosomiasis medicine.drug Follow-Up Studies |
| Zdroj: | Parasite, Vol 12, Iss 4, Pp 353-357 (2005) PARASITE Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1776-1042 |
| Popis: | PCR and FC-ALTA were used to monitor parasite clearance in 54 chronic chagasic patients who had completed therapy with allopurinol (ALLO, n = 31) or itraconazole (ITRA, n = 23) ten years earlier. All patients maintained positive conventional serology. 25 of them showed positive XD (ALLO, n = 11 and ITRA, n = 14) and 29 negative XD (ALLO, n = 20 and ITRA, n = 9). 43 patients were positive by both techniques (ALLO, n = 23 and ITRA, n = 20). Seven of 54 patients were negative by PCR and positive by FC-ALTA and three of 54 were positive by PCR and negative by FC-ALTA. Only one case with both tests negative should be considered cured. Of 29 patients with negative XD, 14 treated ALLO (70 %) and nine with ITRA (77.8 %) showed positive PCR and FC-ALTA. These results do not show differences of efficacy among the drugs, and reinforce the relevance of using sensitive tools such as PCR and FC-ALTA for the follow-up of patients with chronic Chagas disease. |
| Databáze: | OpenAIRE |
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