Nonlinear Optical Microscopy Captures High-Resolution Images of Microstructures Within Three Types of Unlabeled Rat Cartilage
| Autor: | Liqin Zheng, Huang Xinpeng, Jiyang Dong, Xiaoqin Zhu, Jianxin Chen, Shuangmu Zhuo, Hong Lihong, Jianfeng Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
lcsh:Applied optics. Photonics Materials science Elastic cartilage Type (model theory) 01 natural sciences 010309 optics 03 medical and health sciences Optics Nuclear magnetic resonance 0103 physical sciences Microscopy medicine lcsh:QC350-467 Electrical and Electronic Engineering cartilage Hyaline Territorial matrix Hyaline cartilage business.industry second harmonic generation Cartilage nonlinear optical microscopy two-photon excited fluorescence lcsh:TA1501-1820 Atomic and Molecular Physics and Optics 030104 developmental biology medicine.anatomical_structure Fibrocartilage business lcsh:Optics. Light |
| Zdroj: | IEEE Photonics Journal, Vol 8, Iss 3, Pp 1-10 (2016) |
| ISSN: | 1943-0655 |
| Popis: | Cartilage is one of the most important tissues in mammalian growth and is classified into three types: hyaline, fibro, and elastic. To illustrate its potential as a clinical diagnostic tool, nonlinear optical microscopy (NLOM) was applied to simultaneously investigate images and spectra from three different types of rat cartilage. High-resolution images were obtained using the two-photon excited fluorescence and second-harmonic generation (SHG) of structures within fresh specimens. Since samples were imaged without hematoxylin and eosin (H&E) staining, detailed observations of tissue morphology and microstructural distribution were obtained without shrinkage or distortion. Nonlinear optical spectra confirmed that elastic cartilage is rich in elastic fibers, of which there are few in both hyaline and fibro cartilages. Structural dimensions were also measured with this technique, revealing the average area of the lacuna $(398.36 \pm 52.95\ \mu\text{m}^{2})$ , or the average thickness of the territorial matrix $(0.83 \pm 0.26\ \mu\text{m})$ , within elastic cartilage. Fourier analysis of SHG images was performed to further confirm that collagen in fibrocartilage showed significantly greater organization and directionality compared with collagen in hyaline cartilage $(p\ . Our results show that each type of cartilage has different structural features and proportion of matrix, which may significantly contribute to pathology when damaged. Our findings demonstrate that NLOM holds the potential as a clinically relevant diagnostic tool for imaging degenerative tissues or assessing wound repair following cartilage injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|