Daily Gene Expression Rhythms in Rat White Adipose Tissue Do Not Differ Between Subcutaneous and Intra-Abdominal Depots
| Autor: | Andries Kalsbeek, Rianne van der Spek, Susanne E. la Fleur, Eric Fliers |
|---|---|
| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN), AGEM - Endocrinology, metabolism and nutrition, Graduate School, Amsterdam Neuroscience - Cellular & Molecular Mechanisms, Endocrinology, Laboratory for Endocrinology |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Endocrinology Diabetes and Metabolism Period (gene) White adipose tissue lcsh:Diseases of the endocrine glands. Clinical endocrinology 03 medical and health sciences Endocrinology visceral WAT Internal medicine lipid metabolism Journal Article medicine Circadian rhythm Original Research lcsh:RC648-665 biology Leptin nutritional and metabolic diseases PER2 CLOCK circadian 030104 developmental biology biology.protein Resistin retroperitoneal WAT hormones hormone substitutes and hormone antagonists GLUT4 circwave |
| Zdroj: | Frontiers in Endocrinology, 9:206. Frontiers Media SA Frontiers in Endocrinology, Vol 9 (2018) Frontiers in endocrinology, 9(APR):206. Frontiers Media S.A. Frontiers in Endocrinology |
| ISSN: | 1664-2392 |
| DOI: | 10.3389/fendo.2018.00206 |
| Popis: | White adipose tissue (WAT) is present in different depots throughout the body. Although all depots are exposed to systemic humoral signals, they are not functionally identical. Studies in clock gene knockout animals and in shift workers suggest that daily rhythmicity may play an important role in lipid metabolism. Differences in rhythmicity between fat depots might explain differences in depot function; therefore, we measured mRNA expression of clock genes and metabolic genes on a 3-h interval over a 24-h period in the subcutaneous inguinal depot and in the intra-abdominal perirenal, epididymal, and mesenteric depots of male Wistar rats. We analyzed rhythmicity using CircWave software. Additionally, we measured plasma concentrations of glucose, insulin, corticosterone, and leptin. The clock genes (Bmal1/Per2/Cry1/Cry2/RevErba/DBP) showed robust daily gene expression rhythms, which did not vary between WAT depots. Metabolic gene expression rhythms (SREBP1c/PPARα/PPARγ/FAS/LPL/Glut4/HSL/CPT1b/leptin/visfatin/resistin) were more variable between depots. However, no distinct differences between intra-abdominal and subcutaneous rhythms were found. Concluding, specific fat depots are not associated with differences in clock gene expression rhythms and, therefore, do not provide a likely explanation for the differences in metabolic function between different fat depots. |
| Databáze: | OpenAIRE |
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