Osteoclasts Are Present in gp130-Deficient Mice*
| Autor: | Hiromichi Norimatsu, Tatsuo Suda, Toshitaka Nakamura, Kojiro Kawasaki, Kanji Yoshida, Satoshi Yokose, Tadamitsu Kishimoto, Takahito Yuasa, Yoshio Kaji, Hiroko Kataoka, Tetsuya Taga, Yu-Hao Gao, Akira Yamaguchi |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Receptor complex Pathology Osteoclasts Mandible Bone resorption Mice Endocrinology Antigens CD Bone Marrow Reference Values Osteoclast Internal medicine Cytokine Receptor gp130 medicine Animals Membrane Glycoproteins Tibia biology Osteoid Chemistry digestive oral and skin physiology Oncostatin M Glycoprotein 130 Mice Inbred C57BL medicine.anatomical_structure Fibula Gene Targeting biology.protein Bone marrow Tomography X-Ray Computed Leukemia inhibitory factor |
| Zdroj: | Endocrinology. 138:4959-4965 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.138.11.5534 |
| Popis: | Interleukin (IL)-6, IL-11, leukemia inhibitory factor, and oncostatin M similarly induce osteoclast formation in cocultures of osteoblastic cells and bone marrow cells. These cytokines share a common signal transducer, gp130, which forms a receptor complex with the specific receptor for each cytokine. To investigate the role of gp130 in osteoclast development, we examined bone tissues in gp130-deficient and wild-type newborn mice of the ICR background. Soft x-ray radiographs and microfocus x-ray computed tomographs revealed that bone marrow cavities were present in tibiae and radii of both wild-type and gp130-deficient mice. Microfocus x-ray computed tomography and histological examination demonstrated a decrease in the amount of trabeculae at the metaphysial region in tibiae and radii of the gp130-deficient mice compared with the wild-type mice. The number ofosteoclasts in gp130-deficient mice was about double that in the wild-type mice. There were no apparent differences in the distributions of alkaline phosphatase-positive osteoblasts and the osteoid surface on the trabecular bone at the metaphysial region between the wild-type and gp130-deficient mice. The volume of mineralized trabecular bones was also decreased at mandibulae, accompanied by the increased number of osteoclasts in gp130-deficient mice compared with the wild-type and heterozygous mice. These results suggest that the formation of osteoclasts is not solely dependent on gp130 signaling, at least during fetal development. The osteoclastic bone resorption in gp130-deficient mice may be caused by the functional redundancy of bone-resorbing hormones and cytokines other than those of the IL-6 family. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|