Effect of heat stress during in vitro maturation on developmental competence of vitrified bovine oocytes
| Autor: | N. Arcarons, Manel Lopez-Bejar, Teresa Mogas, Meritxell Vendrell-Flotats, E Barau |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0106 biological sciences Embryonic Development Fertilization in Vitro 01 natural sciences Cryopreservation Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology medicine Animals HSP70 Heat-Shock Proteins Vitrification Blastocyst 030219 obstetrics & reproductive medicine urogenital system Chemistry Embryogenesis Temperature Oocyte In vitro In Vitro Oocyte Maturation Techniques Hsp70 In vitro maturation medicine.anatomical_structure embryonic structures Oocytes Cattle Female Animal Science and Zoology Heat-Shock Response 010606 plant biology & botany Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 52:48-51 |
| ISSN: | 0936-6768 |
| DOI: | 10.1111/rda.13055 |
| Popis: | Contents Heat-shock protein 70 (HSP70) plays a crucial role as intracellular cytoprotectant and molecular chaperone. A phenomenon of heat stress (HS) leads to production of these proteins that could be beneficial to cells during cryopreservation, which is also a stressful process for the cell. This study aimed to evaluate the protective effect of exposure of bovine oocytes to moderate HS during in vitro maturation (IVM) prior vitrification. First, oocytes were subjected to HS (41.5°C for 1 hr) at 0, 4, 8, 12 or 16 of IVM. Oocytes in vitro matured for 20 hr served as control group. Presence of HSP70 was detected at 20 hr by immunofluorescence. HSP70 expression was significantly higher when oocytes were subjected to HS at 8 hr of IVM. Next, oocytes were distributed into four groups: Control: IVM oocytes; VIT: oocytes vitrified/warmed at 20 hr of IVM; HS: oocytes subjected to HS at 8 hr of IVM; HS-VIT: oocytes subjected to HS at 8 hr of IVM and vitrified/warmed at 20 hr of IVM. Oocytes were fertilized at 24 hr of IVM, and cleavage and blastocyst yield were assessed. No significant differences were observed among treatments when cleavage rate was evaluated. However, fresh control and HS oocytes resulted in a significantly higher (p |
| Databáze: | OpenAIRE |
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