A Slam-dependent hemophore contributes to heme acquisition in the bacterial pathogen Acinetobacter baumannii
| Autor: | Megha Shah, Hyejin Esther Shin, Thomas J. Bateman, Yogesh Hooda, Trevor F. Moraes, Timothy Pham Ho, Jamie E. Fegan, Scott D. Gray-Owen, Greg Harris, Wangxue Chen, Chuxi Pan, Sang Kyun Ahn, Epshita A. Islam |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Acinetobacter baumannii
Science Serum albumin General Physics and Astronomy Locus (genetics) Heme Biology Bacterial physiology General Biochemistry Genetics and Molecular Biology Article Microbiology chemistry.chemical_compound Bacterial Proteins Membrane proteins Animals Humans Receptor Pathogen Gene Mice Inbred BALB C Multidisciplinary Biological Transport Bacteriology General Chemistry biology.organism_classification chemistry Multigene Family biology.protein Female Hemoglobin Pathogens Acinetobacter Infections |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Nutrient acquisition systems are often crucial for pathogen growth and survival during infection, and represent attractive therapeutic targets. Here, we study the protein machinery required for heme uptake in the opportunistic pathogen Acinetobacter baumannii. We show that the hemO locus, which includes a gene encoding the heme-degrading enzyme, is required for high-affinity heme acquisition from hemoglobin and serum albumin. The hemO locus includes a gene coding for a heme scavenger (HphA), which is secreted by a Slam protein. Furthermore, heme uptake is dependent on a TonB-dependent receptor (HphR), which is important for survival and/or dissemination into the vasculature in a mouse model of pulmonary infection. Our results indicate that A. baumannii uses a two-component receptor system for the acquisition of heme from host heme reservoirs. Nutrient acquisition is crucial for the growth of pathogens within their hosts. Here, Bateman et al. identify a set of proteins required for heme uptake from host hemoglobin and serum albumin in the pathogen Acinetobacter baumannii. |
| Databáze: | OpenAIRE |
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