Decrease lysyl oxidase activity in hearts of copper-deficient bovines
| Autor: | Laura Beatriz Valdez, Leonardo Minatel, Gabriela Cintia Postma, Ivana Agustina Rukavina Mikusic, Andrés Grecco, Carolina Natalia Nicastro |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
chemistry.chemical_element Lysyl oxidase 010501 environmental sciences 01 natural sciences Biochemistry Cofactor Inorganic Chemistry Pathogenesis Protein-Lysine 6-Oxidase 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals 0105 earth and related environmental sciences chemistry.chemical_classification Molybdenum biology Heart medicine.disease Copper Enzyme assay Diet Enzyme Endocrinology chemistry biology.protein Molecular Medicine Cattle Copper deficiency Elastin 030217 neurology & neurosurgery Sulfur |
| Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 65 |
| ISSN: | 1878-3252 |
| Popis: | Background Lysyl oxidase (LOX) is a metalloenzyme that requires Cu as a cofactor and it is responsible for the formation of collagen and elastin cross-linking. The objective of this work was to measure the LOX enzyme activity in the heart of bovines with Cu deficiency induced by high molybdenum and sulfur levels in the diet. Methods Eighteen myocardial samples were obtained from Cu-deficient (n = 9) and control (n = 9) Holstein bovines during two similar assays. The samples were frozen in liquid nitrogen and stored at −70 °C to measure enzymatic activity. A commercial kit was used, following producer instructions. Results The results showed that LOX activity from the hearts of Cu-deficient bovines is 29 % lower than the ones of control bovines, being this difference statistically significant (p = 0.03). Conclusion To our knowledge, this is the first report that determined LOX enzymatic activity in bovine heart of Cu-deficient animals. The microscopic alterations found in these animals in our previous work, could be explained by a diminished LOX activity. The results are in agreement with other authors, who found a relationship between LOX activity and dietary Cu intake. The information provided by this work could help to clarify the pathogenesis of cardiac lesions in cattle with dietary Cu deficiency. |
| Databáze: | OpenAIRE |
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