Origin, migration and fate of newly generated neurons in the adult rodent piriform cortex
| Autor: | R. Kinyamu, Eldon E. Geisert, Mathew Blurton-Jones, Charles E. Ribak, Kwan L. Ng, Lee A. Shapiro, Qun-Yong Zhou, Patricia M. Whitaker-Azmitia |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Doublecortin Domain Proteins
Male Olfactory system Doublecortin Protein Time Factors Histology Cell Survival Nerve Tissue Proteins Biology Rats Sprague-Dawley White matter Mice chemistry.chemical_compound Cell Movement Piriform cortex medicine Animals Cell Lineage Microscopy Immunoelectron Cell Shape Fluorescent Dyes Neurons Microscopy Confocal Staining and Labeling General Neuroscience Neuropeptides Neurogenesis Nuclear Proteins Cell Differentiation Olfactory Pathways Carbocyanines Olfactory Bulb Rats Doublecortin Cell biology DNA-Binding Proteins Ki-67 Antigen medicine.anatomical_structure Animals Newborn Bromodeoxyuridine nervous system chemistry biology.protein Anatomy NeuN Microtubule-Associated Proteins Olfactory epithelium Neuroscience |
| Zdroj: | Brain Structure and Function. 212:133-148 |
| ISSN: | 1863-2661 1863-2653 |
| DOI: | 10.1007/s00429-007-0151-3 |
| Popis: | Newly generated neurons are continuously added to the olfactory epithelium and olfactory bulbs of adult mammals. Studies also report newly generated neurons in the piriform cortex, the primary cortical projection site of the olfactory bulbs. The current study used BrdU-injection paradigms, and in vivo and in vitro DiI tracing methods to address three fundamental issues of these cells: their origin, migratory route and fate. The results show that 1 day after a BrdU-injection, BrdU/DCX double-labeled cells appear deep to the ventricular subependyma, within the white matter. Such cells appear further ventral and caudal in the ensuing days, first appearing in the rostral piriform cortex of mice at 2 days after the BrdU-injection, and at 4 days in the rat. In the caudal piriform cortex, BrdU/DCX labeled cells first appear at 4 days after the injection in mice and 7 days in rats. The time it takes for these cells to appear in the piriform cortex and the temporal distribution pattern suggest that they migrate from outside this region. DiI tracing methods confirmed a migratory route to the piriform cortex from the ventricular subependyma. The presence of BrdU/NeuN labeled cells as early as 7 days after a BrdU injection in mice and 10 days in the rat and lasting as long as 41 days indicates that some of these cells have extended survival durations in the adult piriform cortex. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink