RT-LAMP assay: an alternative approach for profiling of bovine heat shock protein 70 gene in PBMC cultured model
| Autor: | Rani Singh, T. V. Raja, Gyanendra Singh Sengar, Rajiv Kant, Rani Alex, R. R. Alyethodi, Soumendu Chakraborty, Preetam Verma, Sushil Kumar, V. Bhanuprakash, Rajib Deb, Umesh Singh |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Loop-mediated isothermal amplification Gene Expression Biology Real-Time Polymerase Chain Reaction Peripheral blood mononuclear cell Absorbance 03 medical and health sciences Complementary DNA Genetics Animals HSP70 Heat-Shock Proteins RNA Messenger Molecular Biology Cells Cultured 030102 biochemistry & molecular biology General Medicine Molecular biology Fold change Hsp70 030104 developmental biology Real-time polymerase chain reaction Cell culture Leukocytes Mononuclear Cattle Nucleic Acid Amplification Techniques |
| Zdroj: | Molecular Biology Reports. 44:281-287 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-017-4108-1 |
| Popis: | The purpose of this study is to develop a novel Reverse Transcriptase Loop-mediated isothermal amplification (RT-LAMP) based assay for in vitro profiling of heat shock protein 70 (Hsp70) in bovine peripheral blood mononuclear cell (PBMC) culture model utilizing the absorbance level of magnesium pyrophosphate—a by-product of LAMP reaction. A set of bovine Hsp70 specific RT-LAMP primers were designed to detect the differential absorbance level of magnesium pyrophosphate by-product which signifies the degree of Hsp70 amplification from cDNA of thermally induced cultured cells at different recovery periods. The study revealed significant (P |
| Databáze: | OpenAIRE |
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