Tendons from Non-diabetic Humans and Rats Harbor a Population of Insulin-producing, Pancreatic Beta Cell-like Cells
| Autor: | Peter Augat, Daniel Stephan, Hans-Christian Bauer, Corinna Hirzinger, Francisco J. Rivera, Ludwig Aigner, Herbert Tempfer, Andrea Wagner, Herbert Resch, Renate Gehwolf, Christine Lehner |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Cell type Endocrinology Diabetes and Metabolism medicine.medical_treatment Blotting Western Clinical Biochemistry Population Achilles Tendon Biochemistry Tendons Young Adult Endocrinology Insulin-Secreting Cells Internal medicine Diabetes mellitus Insulin Secretion Diabetes Mellitus medicine Animals Humans Insulin education Aged education.field_of_study business.industry Biochemistry (medical) General Medicine Middle Aged Streptozotocin medicine.disease Immunohistochemistry Rats Tendon Glucose medicine.anatomical_structure Female Beta cell Tendinopathy business medicine.drug |
| Zdroj: | Hormone and Metabolic Research. 44:506-510 |
| ISSN: | 1439-4286 0018-5043 |
| Popis: | Diabetes mellitus is a risk factor for various types of tendon disorders. The mechanisms underlying diabetes associated tendinopathies remain unclear, but typically, systemic factors related to high blood glucose levels are thought to be causally involved. We hypothesize that tendon immanent cells might be directly involved in diabetic tendinopathy. We therefore analyzed human and rat tendons by immunohistochemistry, laser capture microdissection, and single cell PCR for pancreatic β-cell associated markers. Moreover, we examined the short term effects of a single injection of streptozotocin, a toxin for GLUT2 expressing cells, in rats on insulin expression of tendon cells, and on the biomechanical properties of Achilles tendons. Tendon cells, both in the perivascular area and in the dense collagenous tissue express insulin and Glut2 on both protein and mRNA levels. In addition, glucagon and PDX-1 are present in tendon cells. Intraperitoneal injection of streptozotocin caused a loss of insulin and insulin mRNA in rat Achilles tendons after only 5 days, accompanied by a 40% reduction of mechanical strength. In summary, a so far unrecognized, extrapancreatic, insulin-producing cell type, possibly playing a major role in the pathophysiology of diabetic tendinopathy is described. In view of these data, novel strategies in tendon repair may be considered. The potential of the described cells as a tool for treating diabetes needs to be addressed by further studies. |
| Databáze: | OpenAIRE |
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