HIV-1 and influenza antigens synthetically linked to IgG2a Fc elicit superior humoral responses compared to unmodified antigens in mice
Autor: | Craig S. Pace, Jian Yu, Yang Song, Yaoxing Huang, David D. Ho, Gerasimos J. Zaharatos, Sandhya Vasan |
---|---|
Rok vydání: | 2011 |
Předmět: |
Recombinant Fusion Proteins
Biology Antibodies Viral medicine.disease_cause Immunoglobulin G Subclass Mice Immune system Adjuvants Immunologic Antigen Influenza A virus medicine Animals Antigens Viral AIDS Vaccines Mice Inbred BALB C General Veterinary General Immunology and Microbiology Effector Immunogenicity Immunoglobulin Fc Fragments Public Health Environmental and Occupational Health Virology Mice Inbred C57BL Infectious Diseases Influenza Vaccines Immunology HIV-1 biology.protein Molecular Medicine |
Zdroj: | Vaccine. 30:42-50 |
ISSN: | 0264-410X |
Popis: | Using murine IgG subclass molecules (IgG1 or IgG2a) synthetically fused to HIV-1 or influenza test antigens, we explored the potential for IgG Fc scaffolds to augment immunogenicity. Each antigen (Ag) was grafted onto a hinge-Fc scaffold containing all critical residues necessary for interaction with effector cells, thus retaining effector functions of the native IgG subclass. We hypothesized that the differential affinity of FcγRs for specific IgG subclasses would influence the magnitude of immune responses elicited by immunization with an Ag-IgG Fc fusion vaccine. We demonstrate here that the antigen-specific humoral response elicited by Ag-IgG2a fusion vaccines is at least tenfold greater than that elicited by native antigen, that this response is superior to that elicited by Ag-IgG1, and that the augmented antigen-specific humoral response elicited is Fcγ receptor-dependent. |
Databáze: | OpenAIRE |
Externí odkaz: |