Plasma Protein Thyroid Hormone Complexes: Separation by Continuous Flow Paper Electrophoresis
| Autor: | Marta Cancio, Efraín Toro-Goyco |
|---|---|
| Rok vydání: | 1963 |
| Předmět: |
medicine.medical_specialty
Globulin Beta-Globulins Serum albumin Fractionation Beta globulins General Biochemistry Genetics and Molecular Biology Iodine Isotopes Internal medicine medicine Electrophoresis Paper Serum Albumin Chromatography biology Chemistry Research Thyroid Gamma globulin Blood Proteins Blood Protein Electrophoresis Blood proteins Thyroxine Cholesterol Endocrinology medicine.anatomical_structure biology.protein Serum Globulins gamma-Globulins Hormone |
| Zdroj: | Experimental Biology and Medicine. 114:297-301 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-114-28657 |
| Popis: | SummaryNine serum protein fractionation experiments using the continuous flow paper electrophoresis technique were performed. Six experiments were performed using the serum collected from individual patients 24 and 48 hours after receiving therapeutic doses of I-131 and 3 using the serum of euthyroid individuals which was incubated in vitro for 1/2 hour at 37°C with I–131 labelled thyroxine prior to fractionation. Protein fractions obtained were identified by conventional paper electrophoresis and analyzed for radioactivity, total protein and cholesterol, the latter being taken as an index of lipid contents. Similar results were obtained for both in vivo and in vitro experiments. Beta globulins were found to be capable of forming thyroid hormone protein complexes to a larger extent than gamma globulins, but the largest amount of radioactive hormone was found as an alpha-2 globulin-thyroid hormone complex. A patient showing a marked abnormality in his plasma proteins showed the highest percent of radioacti... |
| Databáze: | OpenAIRE |
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