Performance characteristics of molecular (DNA) analysis for the diagnosis of mucinous pancreatic cysts
| Autor: | Lee McHenry, Gregory A. Cote, John M. DeWitt, C. Max Schmidt, Leticia P. Luz, Stuart Sherman, Abdul Hamid El Chafic, Julia K. Leblanc, Christen Klochan, Jennifer S. Stuart, Cynthia S. Johnson, Mohammad A. Al-Haddad, Thomas F. Imperiale |
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| Rok vydání: | 2014 |
| Předmět: |
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medicine.medical_specialty Pathology Loss of Heterozygosity Adenocarcinoma Sensitivity and Specificity Surgical pathology Carcinoembryonic antigen Cytology medicine Humans Radiology Nuclear Medicine and imaging Cyst Prospective Studies Prospective cohort study Endoscopic Ultrasound-Guided Fine Needle Aspiration Aged biology Intraductal papillary mucinous neoplasm business.industry Cyst Fluid Significant difference Gastroenterology DNA Middle Aged medicine.disease Carcinoembryonic Antigen Pancreatic Neoplasms Neuroendocrine Tumors Genes ras Mutation biology.protein Female Radiology Pancreatic Cyst Pancreatic cysts business |
| Zdroj: | Gastrointestinal Endoscopy. 79:79-87 |
| ISSN: | 0016-5107 |
| Popis: | Background Diagnosis of mucinous pancreatic cysts (MPCs) is challenging due to the poor sensitivity of cytology provided by EUS-guided-FNA (EUS-FNA). Objective To quantify the test characteristics of molecular (DNA) analysis in suspected low-risk MPCs. Design A prospective cohort study performed in between 2008 and 2011. Setting Academic referral center. Patients Consecutive patients who underwent EUS-FNA of suspected MPCs. Intervention EUS-FNA and molecular (DNA) analysis of cyst fluid. Main Outcome Measurements The sensitivity and specificity of molecular analysis in the diagnosis of MPCs using the criterion standard of surgical pathology in resected cysts. Results Patients with suspected MPCs underwent EUS-FNA and cyst fluid DNA analysis. Surgical resection was performed in 48 patients (17%), confirming a mucinous pathology in 38 (79%). In this group, molecular analysis had a sensitivity of 50% and a specificity of 80% in identifying MPCs (accuracy of 56.3%). The combination of molecular analysis with cyst fluid carcinoembryonic antigen (CEA) and cytology resulted in higher MPC diagnostic performance than either one of its individual components, with a sensitivity, specificity, and accuracy of 73.7%, 70%, and 72.9%, respectively. There was no significant difference in accuracy between molecular analysis and CEA/cytology in this group. Limitations Single-center experience. Conclusion Molecular analysis aids in the diagnosis of MPCs when cytology is nondiagnostic or cyst fluid is insufficient for CEA or its level is indeterminate. Our results do not support the routine use of molecular analysis, which should be used selectively after review of imaging findings and cyst fluid studies. Further studies are needed to assess DNA's performance in malignant cysts. |
| Databáze: | OpenAIRE |
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