Silver (I) as DNA glue: Ag(+)-mediated guanine pairing revealed by removing Watson-Crick constraints
| Autor: | Leonardo Andres Espinosa Leal, Elisabeth G. Gwinn, Olga Lopez-Acevedo, James G. Pavlovich, Steven M. Swasey |
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| Přispěvatelé: | Perustieteiden korkeakoulu, School of Science, Teknillisen fysiikan laitos, Department of Applied Physics, Computational Soft and Molecular Matter (CSMM), Aalto-yliopisto, Aalto University |
| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Circular dichroism Spectrometry Mass Electrospray Ionization Guanine Silver Base pair education ta221 Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid Article Biomaterials chemistry.chemical_compound Cytosine Models DNA nanotechnology Nanotechnology Base Pairing ta218 Genetics Multidisciplinary ta214 ta114 Spectrometry Physics Circular Dichroism Electrospray Ionization Molecular DNA Mass Other Physical Sciences Crystallography chemistry Pairing Nucleic Acid Conformation Thermodynamics Biochemistry and Cell Biology Biotechnology |
| Zdroj: | Scientific Reports Scientific reports, vol 5, iss 1 Swasey, SM; Leal, LE; Lopez-Acevedo, O; Pavlovich, J; & Gwinn, EG. (2015). Silver (I) as DNA glue: Ag+-mediated guanine pairing revealed by removing Watson-Crick constraints. SCIENTIFIC REPORTS, 5. doi: 10.1038/srep10163. UC Santa Barbara: Retrieved from: http://www.escholarship.org/uc/item/63c6z3xw |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep10163. |
| Popis: | Metal ion interactions with DNA have far-reaching implications in biochemistry and DNA nanotechnology. Ag+ is uniquely interesting because it binds exclusively to the bases rather than the backbone of DNA, without the toxicity of Hg2+. In contrast to prior studies of Ag+ incorporation into double-stranded DNA, we remove the constraints of Watson-Crick pairing by focusing on homo-base DNA oligomers of the canonical bases. High resolution electro-spray ionization mass spectrometry reveals an unanticipated Ag+-mediated pairing of guanine homo-base strands, with higher stability than canonical guanine-cytosine pairing. By exploring unrestricted binding geometries, quantum chemical calculations find that Ag+ bridges between non-canonical sites on guanine bases. Circular dichroism spectroscopy shows that the Ag+-mediated structuring of guanine homobase strands persists to at least 90 °C under conditions for which canonical guanine-cytosine duplexes melt below 20 °C. These findings are promising for DNA nanotechnology and metal-ion based biomedical science. |
| Databáze: | OpenAIRE |
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