Membrane-facilitated bioproduction of 3-methylcatechol in an octanol/water two-phase system
| Autor: | Leonie E. Hüsken, Rik Beeftink, Jan A.M. de Bont, Karin Schroën, Mirjam Oomes, Johannes Tramper |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Octanol
Octanols Bio Process Engineering Surface Properties Catechols Bioengineering Polypropylenes Applied Microbiology and Biotechnology Models Biological chemistry.chemical_compound Bioreactors Phase (matter) Biomass Food Process Engineering VLAG Chromatography Aqueous solution biology Pseudomonas putida Aqueous two-phase system Water Membranes Artificial General Medicine biology.organism_classification Organic/aqueous two-phase system Bioproduction Solvent 3-Methylcatechol production chemistry Sodium hydroxide Membrane bioreactor Biotechnology Toluene |
| Zdroj: | Journal of Biotechnology, 96, 281-289 Journal of Biotechnology 96 (2002) |
| ISSN: | 0168-1656 |
| Popis: | Bioproduction of 3-methylcatechol from toluene by Pseudomonas putida MC2 was studied in the presence of an additional 1-octanol phase. This solvent was used to supply the substrate and extract the product, in order to keep the aqueous concentrations low. A hollow-fibre membrane kept the octanol and aqueous phase separated to prevent phase toxicity towards the bacterium. Volumetric production rates increased approximately 40% as compared to two-phase 3-methylcatechol production with direct phase contact. Preliminary investigations on downstream processing of 3-methylcatechol showed that 1 M of sodium hydroxide selectively extracted the disodium salt of 3-methylcatechol into an aqueous phase. |
| Databáze: | OpenAIRE |
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