Single layer colloid centrifugation technique improves motility, viability and chromatin integrity of ram spermatozoa after thawing
| Autor: | M. Kosec, P. Klinc, N. Šterbenc, Detlef Rath, S. Klein, Jane M. Morrell |
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| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_treatment Motility Semen Centrifugation Insemination General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology 03 medical and health sciences Colloid 0302 clinical medicine Freezing medicine Animals Colloids Acrosome Insemination Artificial 030219 obstetrics & reproductive medicine Sheep Chemistry Artificial insemination Cell Membrane 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Chromatin Semen Analysis Fertility Sperm Motility Female General Agricultural and Biological Sciences Semen Preservation |
| Zdroj: | Cryobiology. 86 |
| ISSN: | 1090-2392 |
| Popis: | The cell membrane of ram spermatozoa is more sensitive to the freezing process than in other species due to its composition. As a result, the quality and viability of frozen thawed ram spermatozoa are often poor, which together with the specific structure of the ewe's cervix are the main reasons for lower fertility in ewes after intracervical insemination. In the present study we investigated the effects of semen centrifugation through a single layer of a species-specific colloid (Androcoll-O) on post-thaw quality of ram spermatozoa. Motility, viability and morphology were analysed 0, 6, 12 and 24 h after thawing. DNA fragmentation index (%DFI) of the samples was assessed 0 h after thawing, by SCSA™. Membrane and acrosome integrity of spermatozoa were analysed by Sybr-14/PI/PNA test 0 h after thawing. The proportion of motile spermatozoa was significantly higher in SLC - selected samples in comparison to control (not SLC - selected) samples at 0, 6, 12 (P |
| Databáze: | OpenAIRE |
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