Replication of hepatitis C virus in ascitic mononuclear cells with development of distinct viral quasispecies
| Autor: | Chao-Wei Hsu, Chau-Ting Yeh, Peggy Guey-Chi Chen, Yun-Fan Liaw |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
musculoskeletal diseases
Adult Liver Cirrhosis Male Carcinoma Hepatocellular Hepatitis C virus Hepacivirus Molecular Sequence Data Taiwan Viral quasispecies medicine.disease_cause Virus Replication Virus Flaviviridae Genotype Consensus Sequence medicine Immunology and Allergy Humans Amino Acid Sequence Phylogeny Polymorphism Single-Stranded Conformational Aged biology Reverse Transcriptase Polymerase Chain Reaction Liver Neoplasms Ascites Genetic Variation Single-strand conformation polymorphism Hepatitis C Chronic Middle Aged biology.organism_classification Virology Infectious Diseases Leukocytes Mononuclear RNA Viral Female Viral disease |
| Zdroj: | The Journal of infectious diseases. 180(4) |
| ISSN: | 0022-1899 |
| Popis: | To investigate whether distinct compositions of viral quasispecies developed in the ascitic fluid of patients with late-stage chronic hepatitis C virus (HCV) infection, samples of various origins, including ascitic fluid and ascitic mononuclear cells (AMCs), were analyzed by the method of single-strand conformation polymorphism (SSCP). Subsequently, the major species were isolated, sequenced, and subjected to phylogenetic analysis. Of 42 patients analyzed, HCV-RNA was detectable in the AMCs of 25 patients. SSCP analysis indicated that the compositions of viral quasispecies among samples of different origins were markedly different in this group of patients. Phylogenetic analysis revealed that the ascitic-fluid-derived clones were most closely related to the AMC-derived clones. Minus-strand HCV-RNA was detectable in 5 of them. Our data suggest that HCV can replicate in the AMCs of patients with late-stage chronic HCV, which results in the development of distinct viral quasispecies. |
| Databáze: | OpenAIRE |
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