Cloning and expression of a bovine adenosine A1receptor cDNA
| Autor: | Kevin R. Lynch, Joel Linden, Anna S. Robeva, Amy L. Tucker, D. D. D'angelo |
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| Rok vydání: | 1992 |
| Předmět: |
Molecular Sequence Data
Biophysics Purinergic Gene Expression Biology Binding Competitive Biochemistry Cell Line 03 medical and health sciences Adenosine A1 receptor chemistry.chemical_compound 0302 clinical medicine Structural Biology Sequence Homology Nucleic Acid Complementary DNA Genetics Animals G protein-coupled receptor Amino Acid Sequence Cloning Molecular Receptor Molecular Biology 030304 developmental biology N6-Cyclopentyladenosine 0303 health sciences Base Sequence Purinergic receptor Receptors Purinergic DNA Cell Biology Molecular biology Adenosine receptor Recombinant Proteins 3. Good health chemistry RDC7 Cattle 030217 neurology & neurosurgery Adenosine A2B receptor |
| Zdroj: | FEBS Letters. 297:107-111 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(92)80338-h |
| Popis: | A bovine brain adenosine A1 receptor cDNA encoding a 326 amino acid protein has been identified. This cDNA, which encodes a protein greater than 90% identical to analogous rat and dog receptors, was transiently expressed in COS-1 cells. Recombinant receptors exhibited the features of bovine A1 receptors that distinguish it from rat and canine receptors, including subnanomolar Ki for 1,3-dipropyl-8-cyclopentylxanthine, R-phenylisopropyl- adenosine (R-PIA) and xanthine amino conjugate, and the distinct potency order: R-PIA greater than S-PIA much greater than 5'-N-ethylcarboxamidoadenosine greater than 2'-chloroadenosine. The results indicate that the pharmacological differences between A1 adenosine receptors among species result from only minor differences in receptor structures. |
| Databáze: | OpenAIRE |
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