Further studies on a glycolipid formed from dolichyl-D-glucosyl monophosphate

Autor: Luis F. Leloir, Ana I. Cantarella, Hector Carminatti, Jose A. Levy, Armando J. Parodi, Nicolás H. Behrens, Roberto J. Staneloni
Jazyk: angličtina
Rok vydání: 1973
Předmět:
Los Trabajos de Leloir
Time Factors
Swine
Oligosaccharides
purl.org/becyt/ford/1.7 [https]
Borohydrides
Biochemistry
Pyrophosphate
Analytical Chemistry
purl.org/becyt/ford/1 [https]
chemistry.chemical_compound
Staneloni
Roberto J
Concanavalin A
Leloir Investigador
chemistry.chemical_classification
Carbon Isotopes
Cantarella
Ana I
Periodate
General Medicine
Oligosaccharide
Paper chromatography
Liver
Publicaciones
Microsomes
Liver
Acid hydrolysis
Fatty Alcohols
Oxidation-Reduction
Formic acid
Chromatography
Paper
Artículos Científicos
Parodi
Armando J
Tritium
Los Trabajos del Instituto
Glycolipid
Dolichol
Animals
Leloir
Luis F
purl.org/becyt/ford/1.6 [https]
Chromatography
Binding Sites
Levy
José A
Terpenes
Organic Chemistry
Periodic Acid
Glucosephosphates
Rats
Behrens
Nicolás H
chemistry
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Carminatti
Héctor
Glycolipids
Zdroj: Fundación Instituto Leloir. Archivo fondo LFL
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Incubation of liver microsomes with dolichyl- d -glucosyl- 14 C monophosphate led to the labelling of an endogenous acceptor. This compound seems to be also a dolichol derivative. It contains a high-molecular weight oligosaccharide bound to dolichol through a phosphate or pyrophosphate bond. Various treatments of the labelled oligosaccharide afforded further information on its structure: Reduction with sodium borohydride, followed by acid hydrolysis gave only radioactive d -glucose indicating that the labelled d -glucose is not incorporated at the reducing end of the oligosaccharide. The percentage of radioactivity, liberated as formic acid after periodate oxidation, indicates that more than one molecule of d -glucose is incorporated and that at least one of them is inside the oligosaccharide chain. Alkaline treatment of the otherwise neutral oligosaccharide gave two positively charged derivatives which could be neutralized by N -acetylation, indicating the presence of two hexosamine residues. The oligosaccharides isolated from different tissues by the same method as that used for rat liver, were similar as judged by their migration in paper chromatography and by the pattern of products liberated by acetolysis.
Databáze: OpenAIRE
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