Inhibitory effect of lignin during cellulose bioconversion The effect of lignin chemistry on non-productive enzyme adsorption
| Autor: | Stella Rovio, Raquel Martín-Sampedro, Harri Heikkinen, Kristiina Kruus, Tarja Tamminen, Jenni Rahikainen, Kaisa Marjamaa, Orlando J. Rojas |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cellulase binding
Trichoderma reesei Cel7A WOOD Lignin chemistry.chemical_compound Carbohydrate binding module CELLOBIOHYDROLASE-I Organic chemistry Biomass WHEAT-STRAW Waste Management and Disposal Trichoderma reesei Trichoderma chemistry.chemical_classification biology Hydrolysis food and beverages General Medicine TRICHODERMA-REESEI Biochemistry DELIGNIFICATION Protein Binding Environmental Engineering SURFACE Steam explosion pre-treatment lignin Lignocellulosic biomass Bioengineering Cellulase macromolecular substances Polysaccharide complex mixtures THIN-FILMS Enzymatic hydrolysis SOFTWOOD Cellulose STEAM-EXPLOSION PRETREATMENT steam explosion pre-treatment Hydroxyl Radical Renewable Energy Sustainability and the Environment fungi technology industry and agriculture biology.organism_classification carbohydrate binding module cellulase binding Molecular Weight Enzyme binding chemistry trichoderma reesei Cel7A Quartz Crystal Microbalance Techniques biology.protein Adsorption |
| Zdroj: | Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA Rahikainen, J, Martin-Sampedro, R, Heikkinen, H, Rovio, S, Marjamaa, K, Tamminen, T, Rojas, O J & Kruus, K 2013, ' Inhibitory effect of lignin during cellulose bioconversion : The effect of lignin chemistry on non-productive enzyme adsorption ', Bioresource Technology, vol. 133, pp. 270-278 . https://doi.org/10.1016/j.biortech.2013.01.075 INIA: Repositorio de Resultados de Investigación del INIA |
| DOI: | 10.1016/j.biortech.2013.01.075 |
| Popis: | The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption. © 2013 Elsevier Ltd. |
| Databáze: | OpenAIRE |
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