Peripheral position of CCND1 and HER-2/neu oncogenes within chromosome territories in esophageal and gastric cancers non-related to amplification and overexpression

Autor: Eloiza H. Tajara, Lucimari Bizari, Ana Elizabete Silva
Přispěvatelé: Universidade Estadual Paulista (Unesp), Faculdade de Medicina de São José do Rio Preto (FAMERP)
Rok vydání: 2009
Předmět:
Zdroj: SciELO
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Genetics and Molecular Biology, Volume: 32, Issue: 2, Pages: 242-250, Published: 13 MAR 2009
Genetics and Molecular Biology, Vol 32, Iss 2, Pp 242-250 (2009)
Genetics and Molecular Biology v.32 n.2 2009
Genetics and Molecular Biology
Sociedade Brasileira de Genética (SBG)
instacron:SBG
ISSN: 1678-4685
1415-4757
DOI: 10.1590/s1415-47572009005000034
Popis: Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:05:03Z No. of bitstreams: 1 S1415-47572009000200006.pdf: 120739 bytes, checksum: a3e59baa85d261e9bf4334c104f815fb (MD5) Made available in DSpace on 2013-08-22T19:05:03Z (GMT). No. of bitstreams: 1 S1415-47572009000200006.pdf: 120739 bytes, checksum: a3e59baa85d261e9bf4334c104f815fb (MD5) Previous issue date: 2009-01-01 Made available in DSpace on 2013-09-30T20:08:42Z (GMT). No. of bitstreams: 2 S1415-47572009000200006.pdf: 120739 bytes, checksum: a3e59baa85d261e9bf4334c104f815fb (MD5) S1415-47572009000200006.pdf.txt: 38472 bytes, checksum: d15d8876268dc72b8c959aeb28809e11 (MD5) Previous issue date: 2009-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:00:42Z No. of bitstreams: 2 S1415-47572009000200006.pdf: 120739 bytes, checksum: a3e59baa85d261e9bf4334c104f815fb (MD5) S1415-47572009000200006.pdf.txt: 38472 bytes, checksum: d15d8876268dc72b8c959aeb28809e11 (MD5) Made available in DSpace on 2014-05-20T14:00:42Z (GMT). No. of bitstreams: 2 S1415-47572009000200006.pdf: 120739 bytes, checksum: a3e59baa85d261e9bf4334c104f815fb (MD5) S1415-47572009000200006.pdf.txt: 38472 bytes, checksum: d15d8876268dc72b8c959aeb28809e11 (MD5) Previous issue date: 2009-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Interphase chromosomes have been shown to occupy discrete regions of the nucleus denominated chromosome territories (CTs), their active genes being preferentially positioned on the surfaces of these CTs, where they are accessible to transcriptional machinery. By means of FISH (Fluorescence in situ Hybridization), we analyzed the CCND1 and HER-2/neu gene positions within the CTs and their relationship with gene amplification and protein over-expression in esophageal and gastric cancers. The CCND1 and HER-2/Neu genes were more often positioned at the periphery (mean frequency of 60%-83%) of the CTs in tumor tissues of the esophagus and stomach. Moreover, this positioning revealed no association with either gene amplification or the protein over-expression status of these genes, although, in esophageal carcinoma, Kappa statistics showed a moderate agreement between amplification of the CCND1 gene (Kappa = 0.400) and its location within the CT, as well as with over-expression of the corresponding protein (Kappa = 0.444). Thus, our results suggest that gene positioning in interphase chromosomes does not follow a definitive pattern neither does it depend only on gene transcriptional activity. Apparently, this positioning could be both gene- and tissue-specific, and depends on other factors acting together, such as dense-gene, chromosome size, chromatin structure, and the level and stability of its expression. Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Biologia Faculdade de Medicina Departamento de Biologia Molecular Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Biologia
Databáze: OpenAIRE
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