Identification of microRNA signature in the progression of gestational trophoblastic disease
| Autor: | Wei-Bin Wu, Jiu-Ru Zhao, Ya-Xin Wang, Hui-Juan Zhang, Wei-Wei Cheng, Mei Cai |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Carcinogenesis Immunology Down-Regulation Biology medicine.disease_cause Article S Phase Metastasis 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine SOX2 Cell Movement Pregnancy Cell Line Tumor microRNA medicine Humans Neoplasm Invasiveness lcsh:QH573-671 Neoplasm Metastasis Gestational Trophoblastic Disease Cell Proliferation Regulation of gene expression Base Sequence lcsh:Cytology Gestational trophoblastic disease Gene Expression Profiling Choriocarcinoma Reproducibility of Results Hydatidiform Mole Cell Biology medicine.disease Up-Regulation Gene Expression Regulation Neoplastic Gene expression profiling MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Disease Progression Cancer research Female |
| Zdroj: | Cell Death & Disease Cell Death and Disease, Vol 9, Iss 2, Pp 1-15 (2018) |
| ISSN: | 2041-4889 |
| DOI: | 10.1038/s41419-017-0108-2 |
| Popis: | Gestational trophoblastic disease (GTD) encompasses a range of trophoblast-derived disorders. The most common type of GTD is hydatidiform mole (HM). Some of HMs can further develop into malignant gestational trophoblastic neoplasia (GTN). Aberrant expression of microRNA (miRNA) is widely reported to be involved in the initiation and progression of cancers. MiRNA expression profile also has been proved to be the useful signature for diagnosis, staging, prognosis, and response to chemotherapy. Till now, the profile of miRNA in the progression of GTD has not been determined. In this study, a total of 34 GTN and 60 complete HMs (CHM) trophoblastic tissues were collected. By miRNA array screening and qRT-PCR validating, six miRNAs, including miR-370-3p, -371a-5p, -518a-3p, -519d-3p, -520a-3p, and -934, were identified to be differentially expressed in GTN vs. CHM. Functional analyses further proved that miR-371a-5p and miR-518a-3p promoted proliferation, migration, and invasion of choriocarcinoma cells. Moreover, we demonstrated that miR-371a-5p was negatively related to protein levels of its predictive target genes BCCIP, SOX2, and BNIP3L, while miR-518a-3p was negatively related to MST1 and EFNA4. For the first time, we proved that miR-371a-5p and miR-518a-3p directly targeted to 3′-UTR regions of BCCIP and MST1, respectively. Additionally, we found that miR-371a-5p and miR-518a-3p regulated diverse pathways related to tumorigenesis and metastasis in choriocarcinoma cells. The results presented here may offer new clues to the progression of GTD and may provide diagnostic biomarkers for GTN. |
| Databáze: | OpenAIRE |
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