Quantitative analysis of herpes simplex virus type 1-specific memory B cells generated by different routes of infection
| Autor: | Hye Mee Joo, Daisy J. Vanitha, Mark Y. Sangster, Barry T. Rouse |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Memory B cell
Immunization Secondary Spleen Herpesvirus 1 Human Herpes simplex virus Biology medicine.disease_cause Article Immunoglobulin G Mice Peyer's Patches Antibody Specificity Virology medicine Animals Lymphocyte Count B-Lymphocytes Mediastinum Genital tract Herpes Simplex Mice Inbred C57BL medicine.anatomical_structure Immunization Organ Specificity Mediastinal lymph node Antibody response Immunology biology.protein Nasal administration Lymph Nodes Lymph Immunologic Memory |
| Zdroj: | Virology. 360:136-142 |
| ISSN: | 0042-6822 |
| Popis: | We compared the herpes simplex virus type 1 (HSV)-specific memory B cell (MBC) populations generated by footpad and intranasal infection in mice. Both routes of infection generated transient antibody-secreting cell responses in the draining lymph nodes and spleen, and sustained circulating IgG. HSV-specific IgG MBCs, analyzed by limiting dilution assay approximately 8 weeks after infection, were distributed in a range of lymph nodes and in the spleen and Peyer's patches. Overall, the route of infection had little effect on the MBC frequency in each anatomical location. Interestingly, after both routes of infection there was a trend towards preferential MBC accumulation in the mediastinal lymph node. Intravaginal challenge of mice primed by footpad or intranasal infection generated similar secondary IgG responses. Our findings indicate that the widespread dispersion of MBCs to lymphoid tissues throughout the body is largely independent of the route of infection, but may be influenced by tissue-specific factors. |
| Databáze: | OpenAIRE |
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