Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells
| Autor: | Rafael Roesler, Danielly Brufatto Olguins, Marialva Sinigaglia, André T Brunetto, Lauro José Gregianin, Camila Alves da Silva, Algemir Lunardi Brunetto, Leonardo Horbach, Caroline Brunetto de Farias |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Vincristine Doxorrubicina Drug resistance Expressão gênica doxorubicin vincristine 03 medical and health sciences 0302 clinical medicine medicine Doxorubicin Resistência a medicamentos drug resistance Oncogene business.industry Cancer chemoresistance Articles medicine.disease Pediatric cancer pediatric cancer Sarcoma de Ewing Vincristina 030104 developmental biology Oncology Cell culture 030220 oncology & carcinogenesis Cancer research Sarcoma business medicine.drug |
| Zdroj: | Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS Molecular and Clinical Oncology |
| Popis: | Ewing Sarcoma (ES) is a highly aggressive bone and soft tissue childhood cancer. The development of resistance to chemotherapy is common and remains the main cause of treatment failure. We herein evaluated the expression of genes associated with chemotherapy resistance in ES cell lines. A set of genes (CCAR1, TUBA1A, POLDIP2, SMARCA4 and SMARCB1) was data-mined for resistance against doxorubicin and vincristine, which are the standard drugs used in the treatment of patients with ES. The expression of each gene in SK-ES-1 ES cells was reported before and after exposure to a drug resistance-inducing protocol. There was a significant downregulation of CCAR1 and TUBA1A in doxorubicin-resistant cells, with low expression of TUBA1A in vincristine-resistant cells. By contrast, POLDIP2 was significantly upregulated in cells resistant to either drug, and the expression of the SMARCB1 and SMARCA4 genes was upregulated in doxorubicin-resistant cells. These findings indicate that resistance to specific chemotherapeutic agents was accompanied by differential changes in gene expression in ES tumors. |
| Databáze: | OpenAIRE |
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