Characterization of the chemical constituents in Hongjingtian injection by liquid chromatography quadrupole time-of-flight mass spectrometry
| Autor: | Yining Liu, Wu Haibo, Can-Hui Chen, Zongbao Fang, Xingxian Zhang, Shufang Wang, Jiuwen Qiu |
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| Rok vydání: | 2018 |
| Předmět: |
Monoterpene
Clinical Biochemistry Rhodiola wallichiana Mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Drug Discovery Rhodiola Glycosides Quadrupole time of flight Molecular Biology Pharmacology chemistry.chemical_classification Flavonoids Chromatography biology 010401 analytical chemistry Glycoside General Medicine biology.organism_classification 0104 chemical sciences Rhodiola rosea chemistry Chemical constituents Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Monoterpenes Chromatography Liquid Drugs Chinese Herbal |
| Zdroj: | Biomedical chromatography : BMC. 33(3) |
| ISSN: | 1099-0801 |
| Popis: | Hongjingtian injection is made from Rhodiola wallichiana and used in the treatment of stable angina pectoris associated with coronary heart disease. In this study, the chemical constituents in Hongjingtian injection were comprehensively studied using liquid chromatography quadrupole time-of-flight mass spectrometry. A total of 49 compounds were identified or assumed, including 10 organic acids, nine phenylethanoids, 10 phenylpropanoids, two flavonoid glycosides, seven monoterpene glycosides, seven octylglycosides and four other types of compounds. The structures of seven compounds were confirmed by comparing their retention times, MS and UV spectra with the corresponding authentic standards. Amongst the 49 compounds, 35 were firstly found in R. wallichiana, while they have been reported in other species of the genus Rhodiola, including Rhodiola crenulata, Rhodiola sacra, Rhodiola rosea and Rhodiola kirilowii. The possible fragmentation pathways in the mass spectrometry of the major types of compounds are proposed and summarized. Our study demonstrates a rapid method for characterizing the chemical constituents present in the Hongjingtian injection, which could also be applied to the identification of chemical constituents in other TCM formulae containing R. wallichiana. |
| Databáze: | OpenAIRE |
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