Single-cell RNA-seq analysis unveils a prevalent epithelial/mesenchymal hybrid state during mouse organogenesis
| Autor: | Boqiang Hu, Lu Wen, Yuqiong Hu, Hongshan Guo, Fuchou Tang, Xiaoying Fan, Y. J. Mao, Xinglong Wu, Ji Dong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Mesoderm lcsh:QH426-470 Organogenesis Hindbrain Biology Epithelium Transcriptome 03 medical and health sciences Mice Single-cell analysis Epithelial/mesenchymal hybrid state medicine Animals lcsh:QH301-705.5 Single-cell RNA-seq Neurons Sequence Analysis RNA Interactions between mesenchyme and epithelium Gene Expression Profiling Mesenchymal stem cell Epithelial Cells Hematopoietic Stem Cells Cell biology Gene expression profiling Somite lcsh:Genetics 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Single-Cell Analysis Research Article |
| Zdroj: | Genome Biology, Vol 19, Iss 1, Pp 1-20 (2018) Genome Biology |
| DOI: | 10.1186/s13059-018-1416-2 |
| Popis: | Background Organogenesis is crucial for proper organ formation during mammalian embryonic development. However, the similarities and shared features between different organs and the cellular heterogeneity during this process at single-cell resolution remain elusive. Results We perform single-cell RNA sequencing analysis of 1916 individual cells from eight organs and tissues of E9.5 to E11.5 mouse embryos, namely, the forebrain, hindbrain, skin, heart, somite, lung, liver, and intestine. Based on the regulatory activities rather than the expression patterns, all cells analyzed can be well classified into four major groups with epithelial, mesodermal, hematopoietic, and neuronal identities. For different organs within the same group, the similarities and differences of their features and developmental paths are revealed and reconstructed. Conclusions We identify mutual interactions between epithelial and mesenchymal cells and detect epithelial cells with prevalent mesenchymal features during organogenesis, which are similar to the features of intermediate epithelial/mesenchymal cells during tumorigenesis. The comprehensive transcriptome at single-cell resolution profiled in our study paves the way for future mechanistic studies of the gene-regulatory networks governing mammalian organogenesis. Electronic supplementary material The online version of this article (10.1186/s13059-018-1416-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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