Structural organization of a complex family of palindromic repeats in Enterococci
| Autor: | M. Stella Carlomagno, Raffaele Zarrilli, Pier Paolo Di Nocera, Giustina Silvestro, Tullia Bertocco, Eliana De Gregorio |
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| Přispěvatelé: | DE GREGORIO, Eliana, Bertocco, T., Silvestro, G., Carlomagno, MARIA STELLA, Zarrilli, Raffaele, DI NOCERA, Pierpaolo |
| Rok vydání: | 2009 |
| Předmět: |
Transposable element
DNA Bacterial Enterococcus faecium Molecular Sequence Data Microbiology Enterococcus faecalis Genetics palindromic sequence Insertion sequence Repeated sequence Molecular Biology Palindromic sequence biology Base Sequence stem-loop structure Inverted Repeat Sequences transcription terminators biochemical phenomena metabolism and nutrition biology.organism_classification Stop codon miniature insertion sequence Open reading frame genome analysi Sequence Alignment |
| Zdroj: | FEMS microbiology letters. 292(1) |
| ISSN: | 1574-6968 |
| Popis: | Enterococcus faecalis/faecium repeats (EFARs) are miniature insertion sequences spread in the genome of Enterococcus faecalis and Enterococcus faecium. Unit-length repeats measure 165-170 bp and contain two modules (B and T) capable of folding independently into stem-loop sequences, connected by a short, unstructured module J. The E. faecalis elements feature only one type of B, J and T modules. In contrast, the E. faecium elements result from the assembly of different types of B, J and T modules, and may vary in length because they carry multiple B modules. Most EFARs are located close (0-20 bp) to ORF stop codons, and are thus cotranscribed with upstream flanking genes. In both E. faecalis and E. faecium cells, EFAR transcripts accumulate in a strand-dependent fashion. Data suggest that T modules function as bidirectional transcriptional terminators, which provide a 3'-end to gene transcripts spanning B modules, while blocking antisense transcripts coming in from the opposite direction. |
| Databáze: | OpenAIRE |
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