Increased metal tolerance and bioaccumulation of zinc and cadmium in Chlamydomonas reinhardtii expressing a AtHMA4 C-terminal domain protein
| Autor: | Aniefon Ibuot, Rachel E. Webster, Jon K. Pittman, Lorraine E. Williams |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Calcium alginate chemistry.chemical_element Chlamydomonas reinhardtii Bioengineering Zinc 01 natural sciences Applied Microbiology and Biotechnology Metal 03 medical and health sciences chemistry.chemical_compound Bioremediation 010608 biotechnology Transgenes Adenosine Triphosphatases Cadmium biology Arabidopsis Proteins Chemistry Wild type Biosorption Biological Transport Plants Genetically Modified biology.organism_classification Bioaccumulation Biodegradation Environmental 030104 developmental biology Biochemistry visual_art visual_art.visual_art_medium Biophysics Biotechnology |
| Zdroj: | Ibuot, A, Webster, R, Williams, L E & Pittman, J 2020, ' Increased metal tolerance and bioaccumulation of zinc and cadmium in Chlamydomonas reinhardtii expressing a AtHMA4 C-terminal domain protein ', Biotechnology and Bioengineering, vol. 117, no. 10, pp. 2996-3005 . https://doi.org/10.1002/bit.27476 |
| DOI: | 10.1101/2020.02.13.948307 |
| Popis: | The use of microalgal biomass for metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM Cd and 0.3 mM Zn exposure. The transgenic cells showed increased accumulation and internalisation of both metals compared to wild type. AtHMA4 was expressed either as the full-length protein or just the C-terminal tail, which is known to have metal binding sites. Similar Cd and Zn tolerance and accumulation was observed with expression of either the full-length protein or C-terminal domain, suggesting that enhanced metal tolerance was mainly due to increased metal binding rather than metal transport. The effectiveness of the transgenic cells was further examined by immobilisation in calcium alginate to generate microalgal beads that could be added to a metal contaminated solution. Immobilisation maintained metal tolerance, while AtHMA4-expressing cells in alginate showed a concentration-dependent increase in metal biosorption that was significantly greater than alginate beads composed of wild type cells. This demonstrates that expressing AtHMA4 full-length or C-terminus has great potential as a strategy for bioremediation using microalgal biomass. |
| Databáze: | OpenAIRE |
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