Folic acid-functionalized graphene quantum dots: Synthesis, characterization, radiolabeling with radium-223 and antiviral effect against Zika virus infection
| Autor: | Martha Sahylí Ortega, Pijeira, Alan Silva, de Menezes, Pierre Basílio Almeida, Fechine, Syed Qaiser, Shah, Derya, Ilem-Ozdemir, Elvis O, López, Juliana Terzi, Maricato, Daniela Santoro, Rosa, Eduardo, Ricci-Junior, Severino Alves, Junior, Luciana Magalhães Rebelo, Alencar, Ralph, Santos-Oliveira |
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| Rok vydání: | 2022 |
| Předmět: |
GQD-FA
Graphene quantum dots Zika Virus Infection Cytotoxicity Pharmaceutical Science Dna Zika Virus General Medicine Targeted alpha therapy Antiviral Agents Biomedical device Nanoparticle Folic Acid X-Ray-Fluorescence Radium-223 Smart material Platform Drug delivery Quantum Dots Humans Graphite State Biotechnology |
| Zdroj: | European Journal of Pharmaceutics and Biopharmaceutics. 180:91-100 |
| ISSN: | 0939-6411 |
| Popis: | The use of graphene quantum dots as biomedical devices and drug delivery systems has been increasing. The nano-platform of pure carbon has shown unique properties and is approved to be safe for human use. In this study, we successfully produced and characterized folic acid-functionalized graphene quantum dots (GQD-FA) to evaluate their antiviral activity against Zika virus (ZIKV) infection in vitro, and for radiolabeling with the alpha -particle emitting radionuclide radium-223. The in vitro results exhibited the low cytotoxicity of the nanoprobe GQD-FA in Vero E6 cells and the antiviral effect against replication of the ZIKV infection. In addition, our findings demonstrated that functionalization with folic acid doesn't improve the antiviral effect of graphene quantum dots against ZIVK replication in vitro. On the other hand, the radiolabeled nanoprobe 223Ra@GQD-FA was also produced as confirmed by the Energy Dispersive X-Ray Spectroscopy analysis. 223Ra@GQD-FA might expand the application of alpha targeted therapy using radium-223 in folate receptor-overexpressing tumors. Carlos Chagas Filho Foundation for Research Support of Rio de Janeiro State (FAPERJ) [E-26/200.815/2021, E-26/010.000981/2019, E-26/010.002362/2019, E-26/211.269/2021, E-26//211.207/2021, E-26/202.320/2021]; CNPq [301069/2018-2]; National Nuclear Energy Commission [01341.011064/2021-71] This study was funded by Carlos Chagas Filho Foundation for Research Support of Rio de Janeiro State (FAPERJ) (Cientista do Nosso Estado: E-26/200.815/2021; Rede NanoSaude: E-26/010.000981/2019, Pesquisa na UEZO: E-26/010.002362/2019; Tematicos: E-26/211.269/2021, Infraestrutura e Pesquisa na UEZO e UERJ: E-26//211.207/2021, Bolsa de Pos Doutorado Senior (PDS): E-26/202.320/2021) CNPq (Bolsa de Produtividade 1B: 301069/2018-2) to Ralph Santos-Oliveira. National Nuclear Energy Commission (Bolsa de Posdoutorado CNEN: 01341.011064/2021-71) to Martha Sahyli Ortega Pijeira. |
| Databáze: | OpenAIRE |
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