Molecularly pure miktoarm spherical nucleic acids: preparation and usage as a scaffold for abiotic intracellular catalysis

Autor:	Yue Xiao, Tong Wu, Zehong Cheng, Silei Bai, Bohan Zhang, Xiangyu Chao, Yugang Bai, Ke Zhang, Zhiyong Chen
Rok vydání:	2021
Předmět:	Tris Scaffold chemistry.chemical_compound Chemistry Fullerene Nucleic acid Triazole General Chemistry Combinatorial chemistry Intracellular Cycloaddition Catalysis
Zdroj:	Chemical Science
ISSN:	2041-6520
Popis:	We present a fullerene-based strategy that allows the synthesis of molecularly pure miktoarm spherical nucleic acids (SNAs) with diverse structures, which, with post-functionalization, could serve as efficient scaffolds for intracellular catalysis. The SNA structure promotes cell permeability, nucleic acid stability, and catalytic efficiency, making the platform ideal for in cellulo reactions. Consequently, the tris(triazole)-bearing miktoarm SNA was able to effectively mediate intracellular copper-catalyzed alkyne–azide cycloaddition at nanomolar level of copper, and facilitate the same reaction in live zebrafish. Biocompatible nano-constructs, with definite molecular structures and programmable subunits, can potentially be used as biocatalysts with modular functional moieties.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1423827d0d35509e6f3d23741ca37149 https://pubmed.ncbi.nlm.nih.gov/35024108 Zobrazit plný text záznamu