Preparation and testing of stationary phases and modified capillaries for affinity chromatography and affinity capillary electrophoresis of pepsin
| Autor: | Marie Tichá, Alice Vránková, Karel Štulík, Vera Pacakova, Tereza Varilova |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Swine Organic Chemistry Electrophoresis Capillary General Medicine Gastricsin Silicon Dioxide Biochemistry Chromatography Affinity Pepsin A Analytical Chemistry Dissociation constant Electrophoresis Enzyme Capillary electrophoresis Pepsin Affinity chromatography biology.protein Animals Humans Chymase activity Diiodotyrosine |
| Zdroj: | Journal of chromatography. A. 1084(1-2) |
| ISSN: | 0021-9673 |
| Popis: | Three stationary phases have been prepared for affinity liquid chromatography isolation and separation of porcine and human pepsin. The phases contain 3,5-diiodo-L-tyrosine (DIT) bound to the supports HEMA BIO VS, HEMA BIO E and EPOXY TOYOPEARL. These phases have been tested on a model sample of porcine pepsin A and applied to human pepsin. Fractions have been collected and the chymase activity determined in selected analyses. For affinity CE, capillaries have been prepared by modifying the wall with 3-aminopropyltriethoxysilane, followed either by direct binding of DIT, or by binding L-tyrosine that was subsequently iodated. The dissociation constant K(d) has been determined for the pepsin-DIT complex from the changes in the electrophoretic mobilities. |
| Databáze: | OpenAIRE |
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