Cytostretch, an Organ-on-Chip Platform
| Autor: | Christine L. Mummery, William Quiros Solano, Nikolas Gaio, Lijc Lambert Bergers, Ronald Dekker, Anja van de Stolpe, Pasqualina M. Sarro, Berend J. van Meer |
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| Přispěvatelé: | Dermatology, MOVE Research Institute, Microsystems |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Biological signaling micro-grooves Materials science customizable lcsh:Mechanical engineering and machinery 02 engineering and technology Article 03 medical and health sciences organ-on-chip modular platform Cytostretch micro-electrode array through-membrane pores strain gauges stem cells lcsh:TJ1-1570 Electrical and Electronic Engineering business.industry Mechanical Engineering Modular design 021001 nanoscience & nanotechnology 030104 developmental biology Membrane OA-Fund TU Delft Control and Systems Engineering 0210 nano-technology business Actuator Biomedical engineering |
| Zdroj: | MICROMACHINES, 2016(7):120. Multidisciplinary Digital Publishing Institute (MDPI) Micromachines, Vol 7, Iss 7, p 120 (2016) Gaio, N, van Meer, B, Solano, W Q, Bergers, L, van de Stolpe, A, Mummery, C, Sarro, P M & Dekker, R 2016, ' Cytostretch, an Organ-on-Chip Platform ', MICROMACHINES, vol. 2016, no. 7, 120 . https://doi.org/10.3390/mi7070120 Micromachines, 7(7) Micromachines; Volume 7; Issue 7; Pages: 120 Micromachines, 7(7):120. Multidisciplinary Digital Publishing Institute (MDPI) Micromachines |
| ISSN: | 2072-666X |
| DOI: | 10.3390/mi7070120 |
| Popis: | Organ-on-Chips (OOCs) are micro-fabricated devices which are used to culture cells in order to mimic functional units of human organs. The devices are designed to simulate the physiological environment of tissues in vivo. Cells in some types of OOCs can be stimulated in situ by electrical and/or mechanical actuators. These actuations can mimic physiological conditions in real tissue and may include fluid or air flow, or cyclic stretch and strain as they occur in the lung and heart. These conditions similarly affect cultured cells and may influence their ability to respond appropriately to physiological or pathological stimuli. To date, most focus has been on devices specifically designed to culture just one functional unit of a specific organ: Lung alveoli, kidney nephrons or blood vessels, for example. In contrast, the modular Cytostretch membrane platform described here allows OOCs to be customized to different OOC applications. The platform utilizes silicon-based micro-fabrication techniques that allow low-cost, high-volume manufacturing. We describe the platform concept and its modules developed to date. Membrane variants include membranes with (i) through-membrane pores that allow biological signaling molecules to pass between two different tissue compartments; (ii) a stretchable micro-electrode array for electrical monitoring and stimulation; (iii) micro-patterning to promote cell alignment; and (iv) strain gauges to measure changes in substrate stress. This paper presents the fabrication and the proof of functionality for each module of the Cytostretch membrane. The assessment of each additional module demonstrate that a wide range of OOCs can be achieved. |
| Databáze: | OpenAIRE |
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