Detecção de Fusarium spp. e Trichoderma spp. e antagonismo de Trichoderma sp. em soja sob plantio direto

Autor:	Marlove Brião Muniz, Paola Mendes Milanesi, Elena Blume, Manoeli Lupatini, Z. I. Antoniolli, Lia Rejane Silveira Reiniger, Emanuele Junges
Rok vydání:	2013
Předmět:	Fusarium Rhizosphere education.field_of_study biology Population Biological pest control food and beverages biology.organism_classification complex mixtures Horticulture Intergenic region Botany Root rot General Agricultural and Biological Sciences Antagonism education Ribosomal DNA
Zdroj:	Semina: Ciências Agrárias. 34:3219
ISSN:	1679-0359 1676-546X
Popis:	This study aimed i) to quantify the occurrence of Fusarium spp. and Trichoderma spp. in rhizospheric soil, with and without symptoms of Sudden Death Syndrome (SDS) in eight soybean genotypes; ii) morphologically identify isolates of Fusarium spp. from roots with SDS; iii) evaluate the antagonism between Trichoderma spp. and Fusarium spp. isolates from rhizospheric soil and roots from with and without SDS, respectively; and iv) characterize through the ITS1-5.8S-ITS2 region of rDNA the isolates of Trichoderma spp. with better performance in the direct confrontation. The sampling of soil and roots was performed in an experimental area located in Cruz Alta, RS, Brazil. In the laboratory, serial dilutions of soil samples, counting of the number of Colony Forming Units (UFCs/g-1 of rhizospheric soil) were performed as well as isolation for identification of isolates of Fusarium spp. and Trichoderma spp. and testing of direct confrontation. There were significant differences between the population of Trichoderma spp. in the rhizosphere of plants with and without symptoms of SDS. For the population of Fusarium spp., significant difference was observed only in the rhizosphere of plants without symptoms of SDS. In diseased roots the following species were identified: F. solani , F. avenaceum , F. graminearum , F. oxysporum and F. verticillioides . In the test of direct confrontation, eight isolates of Trichoderma spp. achieved the best performance in the antagonism to Fusarium spp. and Trichoderma spp. from areas with symptoms of SDS had a higher control efficiency in vitro . These isolates showed high similarity to the species of T. koningii agregate.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::141db78affce82204af2b13bae2b154f https://doi.org/10.5433/1679-0359.2013v34n6supl1p3219 Zobrazit plný text záznamu