Rapid Analysis of Glucose, Fructose, Sucrose, and Maltose in Honeys from Different Geographic Regions using Fourier Transform Infrared Spectroscopy and Multivariate Analysis
| Autor: | Qing X. Li, Jun Wang, Michael M. Kliks, Soojin Jun, Mel C. Jackson |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Principal Component Analysis
Sucrose Chromatography Analytical chemistry Fructose Honey Maltose Carbohydrate Chemometrics chemistry.chemical_compound Glucose chemistry Spectroscopy Fourier Transform Infrared Partial least squares regression Least-Squares Analysis Fourier transform infrared spectroscopy Sugar Food Science |
| Zdroj: | Journal of Food Science. 75:C208-C214 |
| ISSN: | 1750-3841 0022-1147 |
| DOI: | 10.1111/j.1750-3841.2009.01504.x |
| Popis: | Quantitative analysis of glucose, fructose, sucrose, and maltose in different geographic origin honey samples in the world using the Fourier transform infrared (FTIR) spectroscopy and chemometrics such as partial least squares (PLS) and principal component regression was studied. The calibration series consisted of 45 standard mixtures, which were made up of glucose, fructose, sucrose, and maltose. There were distinct peak variations of all sugar mixtures in the spectral "fingerprint" region between 1500 and 800 cm(-1). The calibration model was successfully validated using 7 synthetic blend sets of sugars. The PLS 2nd-derivative model showed the highest degree of prediction accuracy with a highest R(2) value of 0.999. Along with the canonical variate analysis, the calibration model further validated by high-performance liquid chromatography measurements for commercial honey samples demonstrates that FTIR can qualitatively and quantitatively determine the presence of glucose, fructose, sucrose, and maltose in multiple regional honey samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text