Effect of cuticular waxes compounds from table grapes on growth, germination and gene expression in Botrytis cinerea
| Autor: | Reinaldo Campos-Vargas, Jocelyn Brito-Echeverría, Evelyn Silva-Moreno, Iván Balic, Rubén Polanco, Miguel López, Juan Carlos Rios |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology 01 natural sciences Applied Microbiology and Biotechnology Conidium Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Botany Gene expression Vitis Oleanolic acid Botrytis cinerea Plant Diseases Wax N alkanes biology Plant Extracts fungi Table grape food and beverages General Medicine Spores Fungal biology.organism_classification Horticulture 030104 developmental biology chemistry Germination visual_art Waxes visual_art.visual_art_medium Botrytis 010606 plant biology & botany Biotechnology |
| Zdroj: | World journal of microbiologybiotechnology. 32(5) |
| ISSN: | 1573-0972 |
| Popis: | Botrytis cinerea attacks a broad range of host causing significant economic losses in the worldwide fruit export industry. Hitherto, many studies have focused on the penetration mechanisms used by this phytopathogen, but little is known about the early stages of infection, especially those such as adhesion and germination. The aim of this work was to evaluate the effect of cuticular waxes compounds from table grapes on growth, germination and gene expression of B. cinerea. To accomplish this, growth was analyzed using as substrate n-alkanes extracted from waxes of fresh fruit (table grapes, blueberries and apricots). Subsequently, the main compounds of table grape waxes, oleanolic acid (OA) and n-fatty alcohols, were mixed to generate a matrix on which conidia of B. cinerea were added to assess their effect on germination and expression of bctub, bchtr and bchex genes. B. cinerea B05.10, isolated from grapes, increased its growth on a matrix composed by table grapes n-alkanes in comparison to a matrix made with n-alkanes from apricot or blueberries. Moreover, at 2.5 h, B05.10 germination increased 17 and 33 % in presence of n-alkanes from table grape, in comparison to conditions without alkanes or with blueberries alkanes, respectively. Finally, expression of bchtr and bchex showed a significant increase during the first hour after contact with n-fatty alcohols and OA. In conclusion, B. cinerea displays selectivity towards certain compounds found in host waxes, mainly n-fatty alcohols, which could be a good candidate to control this phytopathogen in early stages of infection. |
| Databáze: | OpenAIRE |
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