Eri1 regulates microRNA homeostasis and mouse lymphocyte development and antiviral function
| Autor: | K. Mark Ansel, Prescott G. Woodruff, Lewis L. Lanier, Sarah Abdul-Wajid, Marisella Panduro, Misha Rajaram, Joshua E. Babiarz, Molly F. Thomas, Vigo Heissmeyer |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
CD4-Positive T-Lymphocytes
Exonucleases Adoptive cell transfer Small RNA Endogeny Cardiorespiratory Medicine and Haematology Inbred C57BL Biochemistry Mice 0302 clinical medicine RNA interference Exoribonuclease Killer Cells 2.1 Biological and endogenous factors Homeostasis Receptor Cells Cultured 0303 health sciences Mice Inbred ICR Cultured Hematology Inbred ICR Adoptive Transfer 3. Good health Cell biology Mutant Strains Killer Cells Natural Infectious Diseases medicine.anatomical_structure Cytomegalovirus Infections Natural Stem Cell Research - Nonembryonic - Non-Human Infection NK Cell Lectin-Like Receptor Subfamily A Biotechnology Cells 1.1 Normal biological development and functioning Clinical Sciences Immunology Bone Marrow Cells Biology Vaccine Related Paediatrics and Reproductive Medicine 03 medical and health sciences Biodefense microRNA Genetics medicine Animals 030304 developmental biology Immunobiology Prevention Inflammatory and immune system Cell Biology Stem Cell Research Mice Mutant Strains Mice Inbred C57BL MicroRNAs Emerging Infectious Diseases Exoribonucleases Bone marrow 030215 immunology |
| Zdroj: | Blood Thomas, MF; Abdul-Wajid, S; Panduro, M; Babiarz, JE; Rajaram, M; Woodruff, P; et al.(2012). Eri1 regulates microRNA homeostasis and mouse lymphocyte development and antiviral function. Blood, 120(1), 130-142. doi: 10.1182/blood-2011-11-394072. UCSF: Retrieved from: http://www.escholarship.org/uc/item/84w6n2t5 Blood, vol 120, iss 1 Blood 120, 130-142 (2012) |
| ISSN: | 0006-4971 |
| DOI: | 10.1182/blood-2011-11-394072 |
| Popis: | Natural killer (NK) cells play a critical role in early host defense to infected and transformed cells. Here, we show that mice deficient in Eri1, a conserved 3′-to- 5′ exoribonuclease that represses RNA interference, have a cell-intrinsic defect in NK-cell development and maturation. Eri1 -/- NK cells displayed delayed acquisition of Ly49 receptors in the bone marrow (BM) and a selective reduction in Ly49D and Ly49H activating receptors in the periphery. Eri1 was required for immune-mediated control of mouse CMV (MCMV) infection. Ly49H + NK cells deficient in Eri1 failed to expand efficiently during MCMV infection, and virus-specific responses were also diminished among Eri1 -/- T cells. We identified miRNAs as the major endogenous small RNA target of Eri1 in mouse lymphocytes. Both NK and T cells deficient in Eri1 displayed a global, sequence-independent increase in miRNA abundance. Ectopic Eri1 expression rescued defective miRNA expression in mature Eri1 -/- T cells. Thus, mouse Eri1 regulates miRNA homeostasis in lymphocytes and is required for normal NK-cell development and antiviral immunity. © 2012 by The American Society of Hematology. |
| Databáze: | OpenAIRE |
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